VCAM1 CLIA Kit
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- 抗原 See all VCAM1 CLIA Kits
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.195 ng/mL - 50 ng/mL
- 最低检测浓度
- 0.195 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Vascular Cell Adhesion Molecule 1 (VCAM1). No significant cross-reactivity or interference between Vascular Cell Adhesion Molecule 1 (VCAM1) and analogues was observed.
- 灵敏度
- 0.03 ng/mL
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Vascular Cell Adhesion Molecule 1 (VCAM1) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all VCAM1 CLIA Kits
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
- 别名
- Vascular Cell Adhesion Molecule 1 (VCAM1 产品)
- 别名
- CD106 CLIA Kit, INCAM-100 CLIA Kit, Vcam-1 CLIA Kit, VCAM1B CLIA Kit, fi76d06 CLIA Kit, wu:fi76d06 CLIA Kit, zgc:158875 CLIA Kit, VCAM1 CLIA Kit, vascular cell adhesion molecule 1 CLIA Kit, vascular cell adhesion molecule 1b CLIA Kit, VCAM1 CLIA Kit, Vcam1 CLIA Kit, vcam1b CLIA Kit, VCAM-1 CLIA Kit
- 背景
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Gene Name: Vascular Cell Adhesion Molecule 1
Gene Aliases: CD106, INCAM-100, L1CAM
- 基因ID
- 7412
- UniProt
- P19320
- 途径
- Carbohydrate Homeostasis
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