VCP ELISA 试剂盒
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- 抗原 See all VCP ELISA试剂盒
- VCP (Valosin Containing Protein (VCP))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Valosin Containing Protein (VCP). No significant cross-reactivity or interference between Valosin Containing Protein (VCP) and analogues was observed.
- 灵敏度
- 0.112 ng/mL
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Valosin Containing Protein (VCP) ELISA Kit
VCP 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Valosin Containing Protein (VCP) ELISA KitVCP 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Valosin Containing Protein (VCP) ELISA KitVCP 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
Valosin Containing Protein (VCP) ELISA KitSmall Sample VCP 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Valosin Containing Protein (VCP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Valosin Containing Protein (VCP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Valosin Containing Protein (VCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Valosin Containing Protein (VCP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Valosin Containing Protein (VCP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Valosin Containing Protein (VCP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all VCP ELISA试剂盒
- VCP (Valosin Containing Protein (VCP))
- 别名
- Valosin Containing Protein (VCP 产品)
- 别名
- ALS14 ELISA Kit, IBMPFD ELISA Kit, TERA ELISA Kit, p97 ELISA Kit, CDC48 ELISA Kit, wu:fd16d05 ELISA Kit, wu:fj63d11 ELISA Kit, p93 ELISA Kit, 3110001E05 ELISA Kit, p97/VCP ELISA Kit, 22.26 ELISA Kit, 7.12 ELISA Kit, Apple ELISA Kit, BcDNA.GM02885 ELISA Kit, BcDNA:GM02885 ELISA Kit, CG2331 ELISA Kit, DmTER94 ELISA Kit, Dmel\CG2331 ELISA Kit, I ELISA Kit, Ter94 ELISA Kit, Tera ELISA Kit, VCP ELISA Kit, VCP ATPase ELISA Kit, VCP/p97 ELISA Kit, Vcp ELISA Kit, anon-WO2004063362.65 ELISA Kit, anon-WO2004063362.67 ELISA Kit, cdc48 ELISA Kit, dTERA ELISA Kit, dVCP ELISA Kit, dmTERA ELISA Kit, dvcp ELISA Kit, l(2)03775 ELISA Kit, l(2)46CFf ELISA Kit, l(2)46CFs ELISA Kit, l(2)46Ch ELISA Kit, pTER94 ELISA Kit, ter94 ELISA Kit, vcp ELISA Kit, FANCG ELISA Kit, valosin containing protein ELISA Kit, valosin containing protein S homeolog ELISA Kit, valosin-containing protein ELISA Kit, CG2331 gene product from transcript CG2331-RA ELISA Kit, zgc:136908 ELISA Kit, transitional endoplasmic reticulum ATPase ELISA Kit, VCP ELISA Kit, vcp.S ELISA Kit, vcp ELISA Kit, Vcp ELISA Kit, Bm1_56585 ELISA Kit, LOC100136246 ELISA Kit, TER94 ELISA Kit, zgc:136908 ELISA Kit, LOC101109680 ELISA Kit
- 背景
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Gene Name: Valosin Containing Protein
Gene Aliases: IBMPFD, p97, TERA, Transitional Endoplasmic Reticulum ATPase, 15S Mg(2+)-ATPase p97 subunit
- 基因ID
- 7415
- UniProt
- P55072
- 途径
- ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway
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