UBE2L3 CLIA Kit
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- 抗原 See all UBE2L3 CLIA Kits
- UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))
- 适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 156.25 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Ubiquitin Conjugating Enzyme E2L3 (UBE2L3). No significant cross-reactivity or interference between Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) and analogues was observed.
- 灵敏度
- 5.28 pg/mL
- Top Product
- Discover our top product UBE2L3 ELISA Kit
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Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) ELISA Kit
UBE2L3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Conjugating Enzyme E2L3 (UBE2L3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ubiquitin Conjugating Enzyme E2L3 (UBE2L3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all UBE2L3 CLIA Kits
- UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))
- 别名
- Ubiquitin Conjugating Enzyme E2L3 (UBE2L3 产品)
- 别名
- E2-F1 CLIA Kit, L-UBC CLIA Kit, UBCH7 CLIA Kit, UbcM4 CLIA Kit, C79827 CLIA Kit, Ubce7 CLIA Kit, ube2l3 CLIA Kit, wu:fc22h11 CLIA Kit, zgc:86727 CLIA Kit, UBE2L3 CLIA Kit, ube2l3l CLIA Kit, wu:fb38h03 CLIA Kit, ubiquitin conjugating enzyme E2 L3 CLIA Kit, ubiquitin-conjugating enzyme E2L 3 CLIA Kit, ubiquitin-conjugating enzyme E2L 3a CLIA Kit, ubiquitin conjugating enzyme E2 L3 S homeolog CLIA Kit, ubiquitin-conjugating enzyme E2L 3b CLIA Kit, UBE2L3 CLIA Kit, Ube2l3 CLIA Kit, ube2l3a CLIA Kit, ube2l3.S CLIA Kit, ube2l3b CLIA Kit
- 背景
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Gene Name: Ubiquitin Conjugating Enzyme E2L3
Gene Aliases: L-UBC, E2-F1, UBCH7, UbcM4, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3
- 基因ID
- 7332
- UniProt
- P68036
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