UBAP2 ELISA 试剂盒
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- 抗原 See all UBAP2 ELISA试剂盒
- UBAP2 (Ubiquitin Associated Protein 2 (UBAP2))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Ubiquitin Associated Protein 2 (UBAP2). No significant cross-reactivity or interference between Ubiquitin Associated Protein 2 (UBAP2) and analogues was observed.
- 灵敏度
- 0.136 ng/mL
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Ubiquitin Associated Protein 2 (UBAP2) ELISA Kit
UBAP2 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Ubiquitin Associated Protein 2 (UBAP2) ELISA KitUBAP2 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Associated Protein 2 (UBAP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Associated Protein 2 (UBAP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Associated Protein 2 (UBAP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin Associated Protein 2 (UBAP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Associated Protein 2 (UBAP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Associated Protein 2 (UBAP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all UBAP2 ELISA试剂盒
- UBAP2 (Ubiquitin Associated Protein 2 (UBAP2))
- 别名
- Ubiquitin Associated Protein 2 (UBAP2 产品)
- 别名
- UBAP-2 ELISA Kit, bA176F3.5 ELISA Kit, AD012W ELISA Kit, zgc:158449 ELISA Kit, MGC132149 ELISA Kit, 1190005K07Rik ELISA Kit, AA408600 ELISA Kit, AU045235 ELISA Kit, mKIAA1491 ELISA Kit, UBAP2Z ELISA Kit, ubiquitin associated protein 2 ELISA Kit, ubiquitin associated protein 2a ELISA Kit, ubiquitin associated protein 2 L homeolog ELISA Kit, ubiquitin-associated protein 2 ELISA Kit, AD012Z ELISA Kit, UBAP2 ELISA Kit, ubap2a ELISA Kit, ubap2.L ELISA Kit, LOC100303669 ELISA Kit, ubap2 ELISA Kit, Ubap2 ELISA Kit, LOC407092 ELISA Kit
- 背景
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Gene Name: Ubiquitin Associated Protein 2
- 基因ID
- 55833
- UniProt
- Q5T6F2
- 途径
- SARS-CoV-2 Protein Interactome
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