TPT1 ELISA 试剂盒
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- 抗原 See all TPT1 ELISA试剂盒
- TPT1 (Tumor Protein, Translationally-Controlled 1 (TPT1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Tumor Protein, Translationally Controlled 1 (TPT1). No significant cross-reactivity or interference between Tumor Protein, Translationally Controlled 1 (TPT1) and analogues was observed.
- 灵敏度
- 0.128 ng/mL
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Tumor Protein, Translationally-Controlled 1 (TPT1) ELISA Kit
TPT1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Tumor Protein, Translationally-Controlled 1 (TPT1) ELISA KitTPT1 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Tumor Protein, Translationally-Controlled 1 (TPT1) ELISA KitTPT1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Tumor Protein, Translationally-Controlled 1 (TPT1) ELISA KitTPT1 适用: 小鼠 Colorimetric Sandwich ELISA 9.37 pg/mL - 600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Protein, Translationally-Controlled 1 (TPT1) ELISA KitTPT1 适用: 大鼠 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Protein, Translationally-Controlled 1 (TPT1) ELISA KitTPT1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Protein, Translationally Controlled 1 (TPT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Protein, Translationally Controlled 1 (TPT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Protein, Translationally Controlled 1 (TPT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Protein, Translationally Controlled 1 (TPT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Protein, Translationally Controlled 1 (TPT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Protein, Translationally Controlled 1 (TPT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all TPT1 ELISA试剂盒
- TPT1 (Tumor Protein, Translationally-Controlled 1 (TPT1))
- 别名
- Tumor Protein, Translationally Controlled 1 (TPT1 产品)
- 别名
- HRF ELISA Kit, TCTP ELISA Kit, p02 ELISA Kit, Trt ELISA Kit, p21 ELISA Kit, p23 ELISA Kit, TPT1 ELISA Kit, TCT1 ELISA Kit, tctp ELISA Kit, fa28a02 ELISA Kit, id:ibd2078 ELISA Kit, wu:fa28a02 ELISA Kit, PCHK23 ELISA Kit, hrf ELISA Kit, MGC79720 ELISA Kit, Tpt1 ELISA Kit, translationally controlled tumor protein ELISA Kit, CG4800 ELISA Kit, Dmel\\CG4800 ELISA Kit, dTCTP ELISA Kit, Tctp ELISA Kit, tumor protein, translationally-controlled 1 ELISA Kit, tumor protein, translationally-controlled 1 L homeolog ELISA Kit, translationally-controlled tumor protein ELISA Kit, translationally controlled tumor protein ELISA Kit, translationally controlled tumor-like protein ELISA Kit, Translationally controlled tumor protein ELISA Kit, translationally-controlled tumor protein homolog ELISA Kit, P23 tumor protein-like ELISA Kit, TPT1 ELISA Kit, Tpt1 ELISA Kit, tpt1 ELISA Kit, tpt1.L ELISA Kit, LOC711798 ELISA Kit, TCTP ELISA Kit, LOC548038 ELISA Kit, Tctp ELISA Kit, LOC108708523 ELISA Kit, LOC109404863 ELISA Kit, LOC105389397 ELISA Kit, LOC102577554 ELISA Kit
- 背景
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Gene Name: Tumor Protein, Translationally Controlled 1
Gene Aliases: HRF, TCTP, p02, p23, Fortilin, Translationally-Controlled Tumor Protein, Histamine-releasing factor
- 基因ID
- 7178
- UniProt
- P13693
- 途径
- Response to Water Deprivation, Regulation of Cell Size, Negative Regulation of intrinsic apoptotic Signaling
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