p63 ELISA 试剂盒
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- 抗原 See all p63 (TP63) ELISA试剂盒
- p63 (TP63) (Tumor Protein P63 (TP63))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Tumor Protein P63 (TP63). No significant cross-reactivity or interference between Tumor Protein P63 (TP63) and analogues was observed.
- 灵敏度
- 0.057 ng/mL
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Protein P63 (TP63). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Protein P63 (TP63). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Protein P63 (TP63), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Protein P63 (TP63) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Protein P63 (TP63) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Protein P63 (TP63) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all p63 (TP63) ELISA试剂盒
- p63 (TP63) (Tumor Protein P63 (TP63))
- 别名
- Tumor Protein P63 (TP63 产品)
- 别名
- AIS ELISA Kit, B(p51A) ELISA Kit, B(p51B) ELISA Kit, EEC3 ELISA Kit, KET ELISA Kit, LMS ELISA Kit, NBP ELISA Kit, OFC8 ELISA Kit, RHS ELISA Kit, SHFM4 ELISA Kit, TP53CP ELISA Kit, TP53L ELISA Kit, TP73L ELISA Kit, p40 ELISA Kit, p51 ELISA Kit, p53CP ELISA Kit, p63 ELISA Kit, p73H ELISA Kit, p73L ELISA Kit, TP63 ELISA Kit, tp63 ELISA Kit, DNp63 ELISA Kit, id:ibd3516 ELISA Kit, tp73l ELISA Kit, wu:fc89f04 ELISA Kit, wu:fk85h07 ELISA Kit, wu:fk88b02 ELISA Kit, Ket ELISA Kit, P73l ELISA Kit, Tp73l ELISA Kit, Trp63 ELISA Kit, TRP63 ELISA Kit, AI462811 ELISA Kit, P51/P63 ELISA Kit, P63 ELISA Kit, Tp63 ELISA Kit, Trp53rp1 ELISA Kit, Xp63 ELISA Kit, ket ELISA Kit, np63 ELISA Kit, tp73l-A ELISA Kit, trp63 ELISA Kit, tumor protein p63 ELISA Kit, transformation related protein 63 ELISA Kit, tumor protein p63 L homeolog ELISA Kit, TP63 ELISA Kit, tp63 ELISA Kit, Tp63 ELISA Kit, Trp63 ELISA Kit, tp63.L ELISA Kit
- 背景
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Gene Name: Tumor Protein P63
Gene Aliases: P63, TP73L, CUSP, EEC3, KET, LMS, OFC8, RHS, SHFM4, TP53CP, Tumor Protein p53-Competing Protein, Chronic ulcerative stomatitis protein, Keratinocyte transcription factor
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