TPSAB1 ELISA 试剂盒
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- 抗原 See all TPSAB1 ELISA试剂盒
- TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Tryptase (TPS). No significant cross-reactivity or interference between Tryptase (TPS) and analogues was observed.
- 灵敏度
- 13.3 pg/mL
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Tryptase alpha/beta 1 (TPSAB1) ELISA Kit
TPSAB1 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum
Tryptase alpha/beta 1 (TPSAB1) ELISA KitTPSAB1 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Tryptase alpha/beta 1 (TPSAB1) ELISA KitTPSAB1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Tryptase alpha/beta 1 (TPSAB1) ELISA KitTPSAB1 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Tryptase alpha/beta 1 (TPSAB1) ELISA KitWide Range TPSAB1 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Tryptase alpha/beta 1 (TPSAB1) ELISA KitTPSAB1 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Urine
Tryptase alpha/beta 1 (TPSAB1) ELISA KitTPSAB1 适用: 犬 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Tryptase alpha/beta 1 (TPSAB1) ELISA KitTPSAB1 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Tryptase alpha/beta 1 (TPSAB1) ELISA KitTPSAB1 适用: 马 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Tryptase alpha/beta 1 (TPSAB1) ELISA KitHigh Sensitivity TPSAB1 适用: 犬 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptase (TPS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tryptase (TPS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tryptase (TPS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tryptase (TPS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tryptase (TPS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tryptase (TPS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all TPSAB1 ELISA试剂盒
- TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))
- 别名
- Tryptase (TPSAB1 产品)
- 别名
- TPS1 ELISA Kit, TPS2 ELISA Kit, TPSB1 ELISA Kit, PMCT7 ELISA Kit, tpsab1 ELISA Kit, tryptaseB ELISA Kit, tryptaseC ELISA Kit, MMCP-7 ELISA Kit, Mcp-7 ELISA Kit, Mcp7 ELISA Kit, Mcpt7 ELISA Kit, rMCP-7 ELISA Kit, AV011504 ELISA Kit, MMCP-6 ELISA Kit, Mcp-6 ELISA Kit, Mcp6 ELISA Kit, Mcpt6 ELISA Kit, tryptase alpha/beta 1 ELISA Kit, tryptase ELISA Kit, tryptase beta 2 (gene/pseudogene) ELISA Kit, tryptase beta 2 ELISA Kit, TPSAB1 ELISA Kit, MCT7 ELISA Kit, tpsab1 ELISA Kit, TPSB2 ELISA Kit, Tpsab1 ELISA Kit, Tpsb2 ELISA Kit
- 背景
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Gene Name: Tryptase
Gene Aliases: TPSAB1, Tryptase Alpha/Beta 1, Tryptase Alpha II, Tryptase Beta I, Tryptase-I, Tryptase-II, Tryptase-III
- UniProt
- Q15661
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