TNNT1 ELISA 试剂盒
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- 抗原 See all TNNT1 ELISA试剂盒
- TNNT1 (Slow Skeletal Troponin T (TNNT1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.62 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.62 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Troponin T Type 1, Slow Skeletal (TNNT1). No significant cross-reactivity or interference between Troponin T Type 1, Slow Skeletal (TNNT1) and analogues was observed.
- 灵敏度
- 6.5 pg/mL
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Slow Skeletal Troponin T (TNNT1) ELISA Kit
TNNT1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Slow Skeletal Troponin T (TNNT1) ELISA KitTNNT1 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Slow Skeletal Troponin T (TNNT1) ELISA KitTNNT1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Slow Skeletal Troponin T (TNNT1) ELISA KitTNNT1 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin T Type 1, Slow Skeletal (TNNT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Troponin T Type 1, Slow Skeletal (TNNT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin T Type 1, Slow Skeletal (TNNT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin T Type 1, Slow Skeletal (TNNT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Troponin T Type 1, Slow Skeletal (TNNT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Troponin T Type 1, Slow Skeletal (TNNT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all TNNT1 ELISA试剂盒
- TNNT1 (Slow Skeletal Troponin T (TNNT1))
- 别名
- Troponin T Type 1, Slow Skeletal (TNNT1 产品)
- 别名
- ANM ELISA Kit, NEM5 ELISA Kit, STNT ELISA Kit, TNT ELISA Kit, TNTS ELISA Kit, TNNI1 ELISA Kit, AW146156 ELISA Kit, Tnt ELISA Kit, sTnT ELISA Kit, ssTnT ELISA Kit, Fang2 ELISA Kit, tnTs ELISA Kit, Tnnt ELISA Kit, zgc:193831 ELISA Kit, zgc:193865 ELISA Kit, troponin T1, slow skeletal type ELISA Kit, troponin I1, slow skeletal type ELISA Kit, troponin T1, skeletal, slow ELISA Kit, troponin T1, slow skeletal type S homeolog ELISA Kit, troponin T type 1 (skeletal, slow) ELISA Kit, TNNT1 ELISA Kit, TNNI1 ELISA Kit, Tnnt1 ELISA Kit, tnnt1.S ELISA Kit, tnnt1 ELISA Kit
- 背景
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Gene Name: Troponin T Type 1, Slow Skeletal
Gene Aliases: ANM, STNT, TNT, TNTS, Slow Skeletal Muscle Troponin T
- 基因ID
- 7138
- UniProt
- P13805
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