TOM70 ELISA 试剂盒
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- 抗原 See all TOM70 (TOMM70A) ELISA试剂盒
- TOM70 (TOMM70A) (Translocase of Outer Mitochondrial Membrane 70 (TOMM70A))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A). No significant cross-reactivity or interference between Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A) and analogues was observed.
- 灵敏度
- 0.101 ng/mL
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Translocase of Outer Mitochondrial Membrane 70 (TOMM70A) ELISA Kit
TOMM70A 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all TOM70 (TOMM70A) ELISA试剂盒
- TOM70 (TOMM70A) (Translocase of Outer Mitochondrial Membrane 70 (TOMM70A))
- 别名
- Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A 产品)
- 别名
- MGC68780 ELISA Kit, TOMM70A ELISA Kit, Tom70 ELISA Kit, KIAA0719 ELISA Kit, MGC73188 ELISA Kit, zgc:73188 ELISA Kit, wu:fj58b04 ELISA Kit, CG6756 ELISA Kit, Dmel\\CG6756 ELISA Kit, dtom70 ELISA Kit, GB13743 ELISA Kit, TOM70 ELISA Kit, 2610044B22Rik ELISA Kit, D16Ium22 ELISA Kit, D16Ium22e ELISA Kit, D16Wsu109e ELISA Kit, mKIAA0719 ELISA Kit, to translocase of outer mitochondrial membrane 70 S homeolog ELISA Kit, translocase of outer mitochondrial membrane 70 ELISA Kit, translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae) ELISA Kit, Translocase of outer membrane 70 ELISA Kit, mitochondrial import receptor subunit TOM70 ELISA Kit, translocase of outer mitochondrial membrane 70 homolog A (yeast) ELISA Kit, tomm70.S ELISA Kit, TOMM70 ELISA Kit, tomm70a ELISA Kit, tomm70 ELISA Kit, Tom70 ELISA Kit, LOC726081 ELISA Kit, Tomm70 ELISA Kit, Tomm70a ELISA Kit
- 背景
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Gene Name: Translocase Of Outer Mitochondrial Membrane 70A
Gene Aliases: TOM70A, Mitochondrial precursor proteins import receptor, Mitochondrial import receptor subunit TOM70, Translocase of outer membrane 70 kDa subunit
- 基因ID
- 9868
- UniProt
- O94826
- 途径
- SARS-CoV-2 Protein Interactome
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