Topoisomerase I ELISA 试剂盒
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- 抗原 See all Topoisomerase I (TOP1) ELISA试剂盒
- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Topoisomerase I (TOP1). No significant cross-reactivity or interference between Topoisomerase I (TOP1) and analogues was observed.
- 灵敏度
- 0.054 ng/mL
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Topoisomerase (DNA) I (TOP1) ELISA Kit
TOP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Topoisomerase (DNA) I (TOP1) ELISA KitTOP1 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Topoisomerase (DNA) I (TOP1) ELISA KitTOP1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Topoisomerase (DNA) I (TOP1) ELISA KitTOP1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Topoisomerase (DNA) I (TOP1) ELISA KitTOP1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Topoisomerase I (TOP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Topoisomerase I (TOP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Topoisomerase I (TOP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Topoisomerase I (TOP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Topoisomerase I (TOP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Topoisomerase I (TOP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Topoisomerase I (TOP1) ELISA试剂盒
- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
- 别名
- Topoisomerase I (TOP1 产品)
- 别名
- CG6146 ELISA Kit, Dmel\\CG6146 ELISA Kit, TOP1 ELISA Kit, TopI ELISA Kit, Topo I ELISA Kit, TopoI ELISA Kit, dTopoI ELISA Kit, l(1)G0134 ELISA Kit, l(1)G0201 ELISA Kit, l(1)G0229 ELISA Kit, l(1)G0278 ELISA Kit, l(1)top1 ELISA Kit, top1 ELISA Kit, topo I ELISA Kit, topoI ELISA Kit, DNA topoisomerase 1 beta ELISA Kit, DNA topoisomerase I alpha ELISA Kit, MGO1 ELISA Kit, MGOUN 1 ELISA Kit, MTE17.1 ELISA Kit, MTE17_1 ELISA Kit, TOP1BETA ELISA Kit, TOPOISOMERASE 1 ELISA Kit, TOPI ELISA Kit, AI467334 ELISA Kit, D130064I21Rik ELISA Kit, Top-1 ELISA Kit, Ab2-086 ELISA Kit, topi ELISA Kit, Topoisomerase 1 ELISA Kit, DNA topoisomerase I ELISA Kit, DNA topoisomerase I alpha ELISA Kit, DNA topoisomerase 1 ELISA Kit, topoisomerase (DNA) I ELISA Kit, DNA topoisomerase I, gene 1 L homeolog ELISA Kit, Top1 ELISA Kit, top1 ELISA Kit, topA ELISA Kit, ZMO1193 ELISA Kit, MRAD2831_RS63095 ELISA Kit, Lferr_0016 ELISA Kit, PC1_2015 ELISA Kit, Za10_0136 ELISA Kit, Gbro_0575 ELISA Kit, Dd586_2108 ELISA Kit, Kvar_3044 ELISA Kit, Alvin_2069 ELISA Kit, Dacet_0849 ELISA Kit, Mrub_1615 ELISA Kit, Arnit_2962 ELISA Kit, Ndas_4953 ELISA Kit, Trad_2377 ELISA Kit, Acear_1653 ELISA Kit, Mahau_0844 ELISA Kit, Mesop_4109 ELISA Kit, Thein_1586 ELISA Kit, Theth_0241 ELISA Kit, TOP1ALPHA ELISA Kit, top-1 ELISA Kit, TOP1 ELISA Kit, top1.1.L ELISA Kit
- 物质类
- Viral Protein
- 背景
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Gene Name: Topoisomerase I
Gene Aliases: TOPI, DNA Topoisomerase 1
- 基因ID
- 7150
- UniProt
- P11387
- 途径
- Caspase Cascade in Apoptosis, Stem Cell Maintenance
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