THRA ELISA 试剂盒
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- 抗原 See all THRA ELISA试剂盒
- THRA (Thyroid Hormone Receptor, alpha (THRA))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5 pg/mL - 4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Thyroid Hormone Receptor Alpha (THRa). No significant cross-reactivity or interference between Thyroid Hormone Receptor Alpha (THRa) and analogues was observed.
- 灵敏度
- 22.5 pg/mL
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Thyroid Hormone Receptor, alpha (THRA) ELISA Kit
THRA 适用: 人 Colorimetric Sandwich ELISA 1.563 mIU/mL - 100 mIU/mL Plasma, Serum, Tissue Homogenate
Thyroid Hormone Receptor, alpha (THRA) ELISA KitTHRA 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Thyroid Hormone Receptor, alpha (THRA) ELISA KitTHRA 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Tissue Homogenate
Thyroid Hormone Receptor, alpha (THRA) ELISA KitTHRA 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroid Hormone Receptor Alpha (THRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyroid Hormone Receptor Alpha (THRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroid Hormone Receptor Alpha (THRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroid Hormone Receptor Alpha (THRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Hormone Receptor Alpha (THRa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Hormone Receptor Alpha (THRa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all THRA ELISA试剂盒
- THRA (Thyroid Hormone Receptor, alpha (THRA))
- 别名
- Thyroid Hormone Receptor Alpha (THRA 产品)
- 别名
- AR7 ELISA Kit, CHNG6 ELISA Kit, EAR7 ELISA Kit, ERB-T-1 ELISA Kit, ERBA ELISA Kit, ERBA1 ELISA Kit, NR1A1 ELISA Kit, THRA1 ELISA Kit, THRA2 ELISA Kit, c-ERBA-1 ELISA Kit, C-ERBA-ALPHA ELISA Kit, TRalpha ELISA Kit, c-erba ELISA Kit, erba ELISA Kit, nr1a1-A ELISA Kit, thra-A ELISA Kit, thra1 ELISA Kit, TR[a]1 ELISA Kit, nr1a1 ELISA Kit, thra ELISA Kit, thral ELISA Kit, tra ELISA Kit, tra1 ELISA Kit, wu:fb59g07 ELISA Kit, Thra1 ELISA Kit, 6430529J03Rik ELISA Kit, AW259572 ELISA Kit, Erba ELISA Kit, Nr1a1 ELISA Kit, Rvr ELISA Kit, T3R[a] ELISA Kit, T3Ralpha ELISA Kit, Thra2 ELISA Kit, C-ERBA ALPHA1 ELISA Kit, Thra ELISA Kit, fTHRalphaA ELISA Kit, nr1a1-a ELISA Kit, thyroid hormone receptor, alpha ELISA Kit, thyroid hormone receptor, alpha L homeolog ELISA Kit, thyroid hormone receptor alpha a ELISA Kit, thyroid hormone receptor alpha ELISA Kit, thyroid hormone receptor alpha-A ELISA Kit, THRA ELISA Kit, thra.L ELISA Kit, thraa ELISA Kit, Thra ELISA Kit, tr-alpha ELISA Kit, LOC109641414 ELISA Kit
- 背景
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Gene Name: Thyroid Hormone Receptor Alpha
Gene Aliases: THR-A, NR1A1, AR7, EAR7, ERB-T1, ERBA1, NR1-A1, THRA1, THRA2, c-ERBA1, Erythroblastic Leukemia Viral Oncogene Homolog, Nuclear Receptor Subfamily 1,Group A,Member 1
- 基因ID
- 7067
- UniProt
- P10827
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Skeletal Muscle Fiber Development
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