TBK1 ELISA 试剂盒
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- 抗原 See all TBK1 ELISA试剂盒
- TBK1 (TANK-Binding Kinase 1 (TBK1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of TANK Binding Kinase 1 (TBK1). No significant cross-reactivity or interference between TANK Binding Kinase 1 (TBK1) and analogues was observed.
- 灵敏度
- 0.124 ng/mL
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TANK-Binding Kinase 1 (TBK1) ELISA Kit
TBK1 适用: 人, 小鼠, 大鼠 pSer172 Colorimetric Sandwich ELISA Cell Culture Lysate
TANK-Binding Kinase 1 (TBK1) ELISA KitTBK1 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
TANK-Binding Kinase 1 (TBK1) ELISA KitTBK1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
TANK-Binding Kinase 1 (TBK1) ELISA KitTBK1 适用: 人, 小鼠, 大鼠 pSer172 Colorimetric Sandwich ELISA Cell Culture Lysate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TANK Binding Kinase 1 (TBK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TANK Binding Kinase 1 (TBK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TANK Binding Kinase 1 (TBK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TANK Binding Kinase 1 (TBK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TANK Binding Kinase 1 (TBK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TANK Binding Kinase 1 (TBK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all TBK1 ELISA试剂盒
- TBK1 (TANK-Binding Kinase 1 (TBK1))
- 别名
- TANK Binding Kinase 1 (TBK1 产品)
- 别名
- nak ELISA Kit, t2k ELISA Kit, TBK1 ELISA Kit, wu:fk70c05 ELISA Kit, zgc:136548 ELISA Kit, NAK ELISA Kit, T2K ELISA Kit, 1200008B05Rik ELISA Kit, AI462036 ELISA Kit, AW048562 ELISA Kit, TANK binding kinase 1 L homeolog ELISA Kit, TANK binding kinase 1 ELISA Kit, TANK-binding kinase 1 ELISA Kit, tbk1.L ELISA Kit, TBK1 ELISA Kit, tbk1 ELISA Kit, Tbk1 ELISA Kit
- 背景
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Gene Name: TANK Binding Kinase 1
Gene Aliases: NAK, T2K, NF-kappa-B-activating kinase, Serine/threonine-protein kinase TBK1
- 基因ID
- 29110
- UniProt
- Q9UHD2
- 途径
- TLR signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome
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