SCD ELISA 试剂盒
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- 抗原 See all SCD ELISA试剂盒
- SCD (Stearoyl-CoA Desaturase (Delta-9-Desaturase) (SCD))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Stearoyl Coenzyme A Desaturase (SCD). No significant cross-reactivity or interference between Stearoyl Coenzyme A Desaturase (SCD) and analogues was observed.
- 灵敏度
- 0.055 ng/mL
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Stearoyl-CoA Desaturase (Delta-9-Desaturase) (SCD) ELISA Kit
SCD 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Stearoyl-CoA Desaturase (Delta-9-Desaturase) (SCD) ELISA KitSCD 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Stearoyl-CoA Desaturase (Delta-9-Desaturase) (SCD) ELISA KitSCD 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Stearoyl-CoA Desaturase (Delta-9-Desaturase) (SCD) ELISA KitSCD 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stearoyl Coenzyme A Desaturase (SCD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stearoyl Coenzyme A Desaturase (SCD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stearoyl Coenzyme A Desaturase (SCD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stearoyl Coenzyme A Desaturase (SCD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stearoyl Coenzyme A Desaturase (SCD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stearoyl Coenzyme A Desaturase (SCD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all SCD ELISA试剂盒
- SCD (Stearoyl-CoA Desaturase (Delta-9-Desaturase) (SCD))
- 别名
- Stearoyl Coenzyme A Desaturase (SCD 产品)
- 别名
- FADS5 ELISA Kit, MSTP008 ELISA Kit, SCD1 ELISA Kit, SCDOS ELISA Kit, AA589638 ELISA Kit, AI265570 ELISA Kit, Scd ELISA Kit, Scd-1 ELISA Kit, ab ELISA Kit, SCD ELISA Kit, fads5 ELISA Kit, scd1 ELISA Kit, scdos ELISA Kit, F27J15.17 ELISA Kit, F27J15_17 ELISA Kit, STOMATAL CYTOKINESIS-DEFECTIVE 1 ELISA Kit, cds2 ELISA Kit, desaturase ELISA Kit, stearoyl-CoA desaturase ELISA Kit, acyl-CoA desaturase ELISA Kit, Acyl-CoA desaturase ELISA Kit, stearoyl-Coenzyme A desaturase 1 ELISA Kit, stearoyl-CoA desaturase (delta-9-desaturase) ELISA Kit, stearoyl-CoA desaturase (delta-9-desaturase) S homeolog ELISA Kit, stomatal cytokinesis defective / SCD1 protein (SCD1) ELISA Kit, acyl-CoA desaturase-like ELISA Kit, SCD ELISA Kit, CIMG_08158 ELISA Kit, VIBHAR_RS23280 ELISA Kit, acod ELISA Kit, Scd1 ELISA Kit, Scd ELISA Kit, scd ELISA Kit, LOC100346561 ELISA Kit, scd.S ELISA Kit, SCD1 ELISA Kit, LOC100719661 ELISA Kit, LOC101835884 ELISA Kit, LOC109101093 ELISA Kit
- 背景
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Gene Name: Stearoyl Coenzyme A Desaturase
Gene Aliases: FADS5, SCDOS, Fatty Acid desaturase 5, Delta-9-Desaturase, Stearoyl-CoA Desaturase Opposite Strand
- 途径
- Brown Fat Cell Differentiation
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