SPHK1 ELISA 试剂盒
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- 抗原 See all SPHK1 ELISA试剂盒
- SPHK1 (Sphingosine Kinase 1 (SPHK1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Sphingosine Kinase 1 (SPHK1). No significant cross-reactivity or interference between Sphingosine Kinase 1 (SPHK1) and analogues was observed.
- 灵敏度
- 0.122 ng/mL
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Sphingosine Kinase 1 (SPHK1) ELISA Kit
SPHK1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Sphingosine Kinase 1 (SPHK1) ELISA KitSPHK1 适用: 人 AA 1-384 Colorimetric Sandwich ELISA
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine Kinase 1 (SPHK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine Kinase 1 (SPHK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine Kinase 1 (SPHK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine Kinase 1 (SPHK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sphingosine Kinase 1 (SPHK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sphingosine Kinase 1 (SPHK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all SPHK1 ELISA试剂盒
- SPHK1 (Sphingosine Kinase 1 (SPHK1))
- 别名
- Sphingosine Kinase 1 (SPHK1 产品)
- 别名
- CG1747 ELISA Kit, Dmel\\CG1747 ELISA Kit, SK1 ELISA Kit, Sphk1 ELISA Kit, SPHK1 ELISA Kit, F18E5.160 ELISA Kit, F18E5_160 ELISA Kit, sphingosine kinase 1 ELISA Kit, SPHK ELISA Kit, 1110006G24Rik ELISA Kit, Sk1 ELISA Kit, Spk1 ELISA Kit, Sphingosine kinase 1 ELISA Kit, sphingosine kinase 1 ELISA Kit, sphingosine kinase 1 L homeolog ELISA Kit, Sk1 ELISA Kit, SPHK1 ELISA Kit, sphk1.L ELISA Kit, Sphk1 ELISA Kit
- 背景
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Gene Name: Sphingosine Kinase 1
- 基因ID
- 8877
- UniProt
- Q9NYA1
- 途径
- VEGF Signaling
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