SNAIL ELISA 试剂盒
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- 抗原 See all SNAIL (SNAI1) ELISA试剂盒
- SNAIL (SNAI1)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Snail Homolog 1 (SNAI1). No significant cross-reactivity or interference between Snail Homolog 1 (SNAI1) and analogues was observed.
- 灵敏度
- 0.063 ng/mL
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SNAIL (SNAI1) ELISA Kit
SNAI1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
SNAIL (SNAI1) ELISA KitSNAI1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Snail Homolog 1 (SNAI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Snail Homolog 1 (SNAI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Snail Homolog 1 (SNAI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Snail Homolog 1 (SNAI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Snail Homolog 1 (SNAI1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Snail Homolog 1 (SNAI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all SNAIL (SNAI1) ELISA试剂盒
- SNAIL (SNAI1)
- 别名
- Snail Homolog 1 (SNAI1 产品)
- 别名
- SLUGH2 ELISA Kit, SNA ELISA Kit, SNAH ELISA Kit, SNAIL ELISA Kit, SNAIL1 ELISA Kit, dJ710H13.1 ELISA Kit, AI194338 ELISA Kit, Sna ELISA Kit, Sna1 ELISA Kit, Snail ELISA Kit, Snail1 ELISA Kit, SNAI1 ELISA Kit, BG:DS01845.1 ELISA Kit, CG3956 ELISA Kit, Dmel\\CG3956 ELISA Kit, Sco ELISA Kit, br28 ELISA Kit, br29 ELISA Kit, l(2)35Db ELISA Kit, l(2)br28 ELISA Kit, l(2)br29 ELISA Kit, l(3)br28 ELISA Kit, l35Db ELISA Kit, sn ELISA Kit, Xsnail ELISA Kit, sna ELISA Kit, snail1 ELISA Kit, xSna ELISA Kit, Snai ELISA Kit, sna2 ELISA Kit, snail2 ELISA Kit, cb643 ELISA Kit, sna-1 ELISA Kit, sna1 ELISA Kit, snai1 ELISA Kit, snail1a ELISA Kit, zgc:109820 ELISA Kit, snail family transcriptional repressor 1 ELISA Kit, snail family zinc finger 1 ELISA Kit, snail homolog 1 (Drosophila) ELISA Kit, snail ELISA Kit, zinc-finger transcription factor Snail ELISA Kit, snail family zinc finger 1 S homeolog ELISA Kit, protein escargot ELISA Kit, transcription factor protein ELISA Kit, snail zinc finger protein ELISA Kit, snail family zinc finger 1b ELISA Kit, snail family zinc finger 1a ELISA Kit, SNAI1 ELISA Kit, Snai1 ELISA Kit, sna ELISA Kit, snail ELISA Kit, snai1.S ELISA Kit, LOC410464 ELISA Kit, snai1b ELISA Kit, snai1a ELISA Kit
- 背景
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Gene Name: Snail Homolog 1
Gene Aliases: SLUGH2, SNA, SNAH, Snail 1 Zinc Finger Protein
- 基因ID
- 6615
- UniProt
- O95863
- 途径
- Negative Regulation of intrinsic apoptotic Signaling
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