SPTLC1 ELISA 试剂盒
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- 抗原 See all SPTLC1 ELISA试剂盒
- SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1). No significant cross-reactivity or interference between Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) and analogues was observed.
- 灵敏度
- 0.054 ng/mL
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serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) ELISA Kit
SPTLC1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) ELISA KitSPTLC1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all SPTLC1 ELISA试剂盒
- SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))
- 别名
- Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1 产品)
- 别名
- ATSPT1 ELISA Kit, SERINE PALMITOYLTRANSFERASE 1 ELISA Kit, serine palmitoyltransferase 1 ELISA Kit, Afu6g00300 ELISA Kit, HSAN1 ELISA Kit, HSN1 ELISA Kit, LBC1 ELISA Kit, LCB1 ELISA Kit, SPT1 ELISA Kit, SPTI ELISA Kit, wu:fc75h04 ELISA Kit, wu:fp41c08 ELISA Kit, zgc:112247 ELISA Kit, AW552086 ELISA Kit, C77762 ELISA Kit, E030036H05 ELISA Kit, Lcb1 ELISA Kit, RGD1306617 ELISA Kit, Spt1 ELISA Kit, serine palmitoyltransferase 1 ELISA Kit, Serine palmitoyltransferase 1 ELISA Kit, serine palmitoyltransferase long chain base subunit 1 ELISA Kit, serine palmitoyltransferase, long chain base subunit 1 ELISA Kit, serine palmitoyltransferase, long chain base subunit 1 L homeolog ELISA Kit, SPT1 ELISA Kit, AFUA_6G00300 ELISA Kit, PAS_FragB_0040 ELISA Kit, SPTLC1 ELISA Kit, sptlc1 ELISA Kit, sptlc1.L ELISA Kit, Sptlc1 ELISA Kit
- 背景
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Gene Name: Serine Palmitoyltransferase, Long Chain Base Subunit 1
Gene Aliases: HSAN, HSAN1, HSN1, LBC1, LCB1, SPT1, SPTI, Hereditary Sensory Neuropathy,Type 1, Long chain base biosynthesis protein 1, Serine-palmitoyl-CoA transferase 1
- 基因ID
- 268656
- UniProt
- O35704
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