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S100A10 ELISA 试剂盒

S100A10 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
产品编号 ABIN5658548
发货至: 中国
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    S100A10 (S100 Calcium Binding Protein A10 (S100A10))
    适用
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.312 ng/mL - 20 ng/mL
    最低检测浓度
    0.312 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A10 (S100A10). No significant cross-reactivity or interference between S100 Calcium Binding Protein A10 (S100A10) and analogues was observed.
    灵敏度
    0.115 ng/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A10 (S100A10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A10 (S100A10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A10 (S100A10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A10 (S100A10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A10 (S100A10) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A10 (S100A10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all S100A10 ELISA试剂盒
    S100A10 (S100 Calcium Binding Protein A10 (S100A10))
    别名
    S100 Calcium Binding Protein A10 (S100A10 产品)
    别名
    42C ELISA Kit, ANX2L ELISA Kit, ANX2LG ELISA Kit, CAL1L ELISA Kit, CLP11 ELISA Kit, Ca[1] ELISA Kit, GP11 ELISA Kit, P11 ELISA Kit, p10 ELISA Kit, AA409961 ELISA Kit, AL024248 ELISA Kit, CAL12 ELISA Kit, Cal1l ELISA Kit, p11 ELISA Kit, s100a10 ELISA Kit, 42c ELISA Kit, anx2l ELISA Kit, anx2lg ELISA Kit, cal1l ELISA Kit, calpactin ELISA Kit, clp11 ELISA Kit, gp11 ELISA Kit, p11-A ELISA Kit, s100a10b ELISA Kit, hm:zehn1116 ELISA Kit, wu:fa94d11 ELISA Kit, wu:fb05h04 ELISA Kit, zgc:56142 ELISA Kit, zgc:101880 ELISA Kit, S100 calcium binding protein A10 ELISA Kit, S100 calcium binding protein A10 (calpactin) ELISA Kit, S100 calcium binding protein A10 L homeolog ELISA Kit, S100 calcium binding protein A10b ELISA Kit, S100 calcium binding protein A10a ELISA Kit, S100A10 ELISA Kit, S100a10 ELISA Kit, s100a10 ELISA Kit, s100a10.L ELISA Kit, s100a10b ELISA Kit, s100a10a ELISA Kit
    背景

    Gene Name: S100 Calcium Binding Protein A10

    Gene Aliases: ANX2L, ANX2LG, CAL1L, CLP11, GP11, P11, P10, Calpactin-1 light chain, Cellular ligand of annexin II, Annexin II Ligand,Calpactin I,Light Polypeptide

    UniProt
    P05943
    途径
    S100 Proteins
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