RHD ELISA 试剂盒
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- 抗原 See all RHD ELISA试剂盒
- RHD (Rh Blood Group, D Antigen (RHD))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 2.47 ng/mL - 200 ng/mL
- 最低检测浓度
- 2.47 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Rh Blood Group, D Antigen (RHD). No significant cross-reactivity or interference between Rh Blood Group, D Antigen (RHD) and analogues was observed.
- 灵敏度
- 0.92 ng/mL
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Rh Blood Group, D Antigen (RHD) ELISA Kit
RHD 适用: 人 Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Rh Blood Group, D Antigen (RHD) ELISA KitRHD 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Rh Blood Group, D Antigen (RHD) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Rh Blood Group, D Antigen (RHD) and unlabeled Rh Blood Group, D Antigen (RHD) (Standards or samples) with the pre-coated antibody specific to Rh Blood Group, D Antigen (RHD). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Rh Blood Group, D Antigen (RHD) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Rh Blood Group, D Antigen (RHD) in the sample.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rh Blood Group, D Antigen (RHD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rh Blood Group, D Antigen (RHD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all RHD ELISA试剂盒
- RHD (Rh Blood Group, D Antigen (RHD))
- 别名
- Rh Blood Group, D Antigen (RHD 产品)
- 别名
- CD240D ELISA Kit, DIIIc ELISA Kit, RH ELISA Kit, RH30 ELISA Kit, RHCED ELISA Kit, RHDVA(TT) ELISA Kit, RHDel ELISA Kit, RHPII ELISA Kit, RHXIII ELISA Kit, Rh4 ELISA Kit, RhDCw ELISA Kit, RhII ELISA Kit, RhK562-II ELISA Kit, RhPI ELISA Kit, Rh ELISA Kit, Rhced ELISA Kit, Rhl1 ELISA Kit, Rh blood group D antigen ELISA Kit, Rh blood group, D antigen ELISA Kit, RHD ELISA Kit, Rhd ELISA Kit
- 背景
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Gene Name: Rh Blood Group, D Antigen
Gene Aliases: CD240D, Rh30a, Rh4, RhPI, RhII, DIIIc, Rhesus D antigen, Rh polypeptide 2
- 基因ID
- 6007
- UniProt
- Q02161
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