RBP3 ELISA 试剂盒
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- RBP3 (Retinol Binding Protein 3, Interstitial (RBP3))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Retinol Binding Protein 3, Interstitial (RBP3). No significant cross-reactivity or interference between Retinol Binding Protein 3, Interstitial (RBP3) and analogues was observed.
- 灵敏度
- 0.055 ng/mL
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Retinol Binding Protein 3, Interstitial (RBP3) ELISA Kit
RBP3 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 3, Interstitial (RBP3) ELISA KitRBP3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Retinol Binding Protein 3, Interstitial (RBP3) ELISA KitRBP3 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinol Binding Protein 3, Interstitial (RBP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinol Binding Protein 3, Interstitial (RBP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinol Binding Protein 3, Interstitial (RBP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinol Binding Protein 3, Interstitial (RBP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinol Binding Protein 3, Interstitial (RBP3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinol Binding Protein 3, Interstitial (RBP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all RBP3 ELISA试剂盒
- RBP3 (Retinol Binding Protein 3, Interstitial (RBP3))
- 别名
- Retinol Binding Protein 3, Interstitial (RBP3 产品)
- 别名
- Irbp ELISA Kit, Rbp-3 ELISA Kit, D10S64 ELISA Kit, D10S65 ELISA Kit, D10S66 ELISA Kit, IRBP ELISA Kit, RBPI ELISA Kit, RP66 ELISA Kit, retinol binding protein 3 ELISA Kit, interphotoreceptor retinoid-binding protein ELISA Kit, retinol binding protein 3, interstitial ELISA Kit, rbp3 ELISA Kit, RBP3 ELISA Kit, glr2547 ELISA Kit, XOO2681 ELISA Kit, AMED_0162 ELISA Kit, ambt_08395 ELISA Kit, Rbp3 ELISA Kit
- 背景
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Gene Name: Retinol Binding Protein 3, Interstitial
Gene Aliases: RBP-3, IRBP, RBPI, Interphotoreceptor retinoid-binding protein, Interstitial retinol-binding protein
- 基因ID
- 5949
- UniProt
- P10745
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