RETNLB ELISA 试剂盒
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- 抗原 See all RETNLB ELISA试剂盒
- RETNLB (Resistin Like beta (RETNLB))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 6.25 pg/mL - 400 pg/mL
- 最低检测浓度
- 6.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Resistin Like Beta (RETNLb). No significant cross-reactivity or interference between Resistin Like Beta (RETNLb) and analogues was observed.
- 灵敏度
- 2.54 pg/mL
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Resistin Like beta (RETNLB) ELISA Kit
RETNLB 适用: 人 Colorimetric Sandwich ELISA 47 pg/mL - 3000 pg/mL Cell Culture Supernatant, Plasma, Serum
Resistin Like beta (RETNLB) ELISA KitRETNLB 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Resistin Like beta (RETNLB) ELISA KitRETNLB 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Resistin Like Beta (RETNLb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Resistin Like Beta (RETNLb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Resistin Like Beta (RETNLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Resistin Like Beta (RETNLb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Resistin Like Beta (RETNLb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Resistin Like Beta (RETNLb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all RETNLB ELISA试剂盒
- RETNLB (Resistin Like beta (RETNLB))
- 别名
- Resistin Like Beta (RETNLB 产品)
- 别名
- FIZZ1 ELISA Kit, FIZZ2 ELISA Kit, HXCP2 ELISA Kit, RELM-beta ELISA Kit, RELMb ELISA Kit, RELMbeta ELISA Kit, XCP2 ELISA Kit, RETNLB ELISA Kit, 9030012B21Rik ELISA Kit, Fizz2 ELISA Kit, Relmb ELISA Kit, Xcp3 ELISA Kit, resistin like beta ELISA Kit, RETNLB ELISA Kit, Retnlb ELISA Kit
- 背景
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Gene Name: Resistin Like Beta
Gene Aliases: XCP2, FIZZ2, HXCP2, CCRG, RETNL2, RELMb, Foundin In Flammatory Zone 2, Colon and small intestine-specific cysteine-rich protein, Colon carcinoma-related gene protein
- 基因ID
- 57263
- UniProt
- Q99P86
- 途径
- Hormone Activity
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