RIPK1 ELISA 试剂盒
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- 抗原 See all RIPK1 ELISA试剂盒
- RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Receptor Interacting Serine Threonine Kinase 1 (RIPK1). No significant cross-reactivity or interference between Receptor Interacting Serine Threonine Kinase 1 (RIPK1) and analogues was observed.
- 灵敏度
- 0.063 ng/mL
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Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) ELISA Kit
RIPK1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) ELISA KitRIPK1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) ELISA KitRIPK1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) ELISA KitRIPK1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) ELISA KitHigh Sensitivity RIPK1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Interacting Serine Threonine Kinase 1 (RIPK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Interacting Serine Threonine Kinase 1 (RIPK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Interacting Serine Threonine Kinase 1 (RIPK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor Interacting Serine Threonine Kinase 1 (RIPK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 1 (RIPK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 1 (RIPK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all RIPK1 ELISA试剂盒
- RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))
- 别名
- Receptor Interacting Serine Threonine Kinase 1 (RIPK1 产品)
- 别名
- RIP ELISA Kit, RIP1 ELISA Kit, D330015H01Rik ELISA Kit, Rinp ELISA Kit, Rip1 ELISA Kit, rip1 ELISA Kit, ripk1 ELISA Kit, xRIP1beta ELISA Kit, xrip1 ELISA Kit, receptor interacting serine/threonine kinase 1 ELISA Kit, receptor (TNFRSF)-interacting serine-threonine kinase 1 ELISA Kit, receptor interacting serine/threonine kinase 1 L homeolog ELISA Kit, RIPK1 ELISA Kit, Ripk1 ELISA Kit, ripk1.L ELISA Kit
- 背景
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Gene Name: Receptor Interacting Serine Threonine Kinase 1
Gene Aliases: RIP, Receptor-Interacting Protein, Cell death protein RIP, Serine/threonine-protein kinase RIP
- 基因ID
- 8737
- UniProt
- Q13546
- 途径
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Activation of Innate immune Response, Inositol Metabolic Process, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, Ubiquitin Proteasome Pathway
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