RACGAP1 ELISA 试剂盒
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- 抗原 See all RACGAP1 ELISA试剂盒
- RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Rac-GTPase Activating Protein 1 (RACGAP1). No significant cross-reactivity or interference between Rac-GTPase Activating Protein 1 (RACGAP1) and analogues was observed.
- 灵敏度
- 0.056 ng/mL
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Rac GTPase Activating Protein 1 (RACGAP1) ELISA Kit
RACGAP1 适用: 人 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Rac GTPase Activating Protein 1 (RACGAP1) ELISA KitRACGAP1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Rac GTPase Activating Protein 1 (RACGAP1) ELISA KitRACGAP1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rac-GTPase Activating Protein 1 (RACGAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rac-GTPase Activating Protein 1 (RACGAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rac-GTPase Activating Protein 1 (RACGAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rac-GTPase Activating Protein 1 (RACGAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rac-GTPase Activating Protein 1 (RACGAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rac-GTPase Activating Protein 1 (RACGAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all RACGAP1 ELISA试剂盒
- RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))
- 别名
- Rac-GTPase Activating Protein 1 (RACGAP1 产品)
- 别名
- CYK4 ELISA Kit, HsCYK-4 ELISA Kit, ID-GAP ELISA Kit, MgcRacGAP ELISA Kit, cyk-4 ELISA Kit, id-gap ELISA Kit, mgcracgap ELISA Kit, RACGAP1 ELISA Kit, MGC121578 ELISA Kit, Gap ELISA Kit, RasGAP ELISA Kit, Rasa ELISA Kit, GAPX ELISA Kit, AI227039 ELISA Kit, AI327394 ELISA Kit, Band25 ELISA Kit, GTPase ELISA Kit, gtl11 ELISA Kit, mKIAA1478 ELISA Kit, wu:fb92c08 ELISA Kit, zgc:77839 ELISA Kit, Rac GTPase activating protein 1 ELISA Kit, rac GTPase-activating protein 1 ELISA Kit, Rac GTPase activating protein 1 L homeolog ELISA Kit, RAS p21 protein activator 1 ELISA Kit, Rac GTPase-activating protein 1 ELISA Kit, si:ch1073-416j23.1 ELISA Kit, RACGAP1 ELISA Kit, racgap1 ELISA Kit, racgap1.L ELISA Kit, racgap1.2.L ELISA Kit, racgap1.2 ELISA Kit, Rasa1 ELISA Kit, Racgap1 ELISA Kit, si:ch1073-416j23.1 ELISA Kit
- 背景
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Gene Name: Rac-GTPase Activating Protein 1
Gene Aliases: HsCYK-4, ID-GAP, MgcRacGAP, Male germ cell RacGap
- 基因ID
- 29127
- UniProt
- Q9H0H5
- 途径
- Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor
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