AKT2 ELISA 试剂盒
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- 抗原 See all AKT2 ELISA试剂盒
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Protein Kinase B Beta (PKBb). No significant cross-reactivity or interference between Protein Kinase B Beta (PKBb) and analogues was observed.
- 灵敏度
- 0.124 ng/mL
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V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) ELISA Kit
AKT2 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) ELISA KitAKT2 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) ELISA KitAKT2 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) ELISA KitAKT2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) ELISA KitAKT2 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase B Beta (PKBb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase B Beta (PKBb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase B Beta (PKBb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase B Beta (PKBb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase B Beta (PKBb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase B Beta (PKBb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all AKT2 ELISA试剂盒
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- 别名
- Protein Kinase B Beta (AKT2 产品)
- 别名
- AKT2 ELISA Kit, pkbb ELISA Kit, pkbbeta ELISA Kit, pkbss ELISA Kit, prkbb ELISA Kit, rac-beta ELISA Kit, v-akt2 ELISA Kit, 2410016A19Rik ELISA Kit, AW554154 ELISA Kit, PKB ELISA Kit, PKBbeta ELISA Kit, HIHGHH ELISA Kit, PKBB ELISA Kit, PKBBETA ELISA Kit, PRKBB ELISA Kit, RAC-BETA ELISA Kit, cb945 ELISA Kit, akt2-a ELISA Kit, AKT serine/threonine kinase 2 ELISA Kit, v-akt murine thymoma viral oncogene homolog 2 ELISA Kit, thymoma viral proto-oncogene 2 ELISA Kit, v-akt murine thymoma viral oncogene homolog 2 L homeolog ELISA Kit, AKT2 ELISA Kit, akt2 ELISA Kit, Akt2 ELISA Kit, akt2.L ELISA Kit
- 背景
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Gene Name: Protein Kinase B Beta
Gene Aliases: AKT2, PKBBETA, PRKB-B, RAC-BETA, RAC-Beta Serine/Threonine-Protein Kinase, V-Akt Murine Thymoma Viral Oncogene Homolog 2
- 基因ID
- 208
- UniProt
- P31751
- 途径
- PI3K-Akt Signaling, RTK signaling, AMPK Signaling, TLR signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
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