PRNP ELISA 试剂盒
-
- 抗原 See all PRNP ELISA试剂盒
- PRNP (Prion Protein (PRNP))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Prion Protein (PRNP). No significant cross-reactivity or interference between Prion Protein (PRNP) and analogues was observed.
- 灵敏度
- 0.055 ng/mL
- Featured
- Discover our best selling PRNP ELISA Kit
- Top Product
- Discover our top product PRNP ELISA Kit
-
Prion Protein (PRNP) ELISA Kit
PRNP 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Prion Protein (PRNP) ELISA KitPRNP 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Prion Protein (PRNP) ELISA KitPRNP 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Prion Protein (PRNP) ELISA KitPRNP 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Prion Protein (PRNP) ELISA KitHigh Sensitivity PRNP 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Prion Protein (PRNP) ELISA KitPRNP 适用: Cow Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Prion Protein (PRNP) ELISA KitPRNP 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Prion Protein (PRNP) ELISA KitSmall Sample PRNP 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prion Protein (PRNP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prion Protein (PRNP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prion Protein (PRNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prion Protein (PRNP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prion Protein (PRNP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prion Protein (PRNP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all PRNP ELISA试剂盒
- PRNP (Prion Protein (PRNP))
- 别名
- Prion Protein (PRNP 产品)
- 别名
-
ASCR ELISA Kit, AltPrP ELISA Kit, CD230 ELISA Kit, CJD ELISA Kit, GSS ELISA Kit, KURU ELISA Kit, PRIP ELISA Kit, PrP ELISA Kit, PrP27-30 ELISA Kit, PrP33-35C ELISA Kit, PrPc ELISA Kit, p27-30 ELISA Kit, Prn ELISA Kit, Prn-p ELISA Kit, prp ELISA Kit, cjd ELISA Kit, prion ELISA Kit, prp(c) ELISA Kit, MGC84114 ELISA Kit, PRP ELISA Kit, PRPC ELISA Kit, Prp ELISA Kit, SIP ELISA Kit, PRNP ELISA Kit, AA960666 ELISA Kit, AI325101 ELISA Kit, PrP
ELISA Kit, PrPC ELISA Kit, PrPSc ELISA Kit, Prn-i ELISA Kit, Sinc ELISA Kit, prP27-30 ELISA Kit, prP33-35C ELISA Kit, PrP-1 ELISA Kit, PrP1 ELISA Kit, prnprs1 ELISA Kit, stPrP-1 ELISA Kit, zgc:123004 ELISA Kit, prion protein ELISA Kit, prion protein S homeolog ELISA Kit, prion protein b ELISA Kit, PRNP ELISA Kit, Prnp ELISA Kit, prnp.S ELISA Kit, prnpb ELISA Kit - 背景
-
Gene Name: Prion Protein
Gene Aliases: CD230, GSS, ASCR, CJD, PRIP, PrP, PrP2730, PrPc, Major prion protein, Creutzfeldt-Jakob Disease, Gerstmann-Strausler-Scheinker Syndrome, Fatal Familial Insomnia
- 基因ID
- 5621
- UniProt
- P04156
- 途径
- Transition Metal Ion Homeostasis, Activated T Cell Proliferation
-
