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PNPLA2 ELISA 试剂盒

PNPLA2 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
产品编号 ABIN5657790
发货至: 中国
  • 抗原 See all PNPLA2 ELISA试剂盒
    PNPLA2 (Calcium Independent Phospholipase A2 (PNPLA2))
    适用
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    78 pg/mL - 5000 pg/mL
    最低检测浓度
    78 pg/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Calcium Independent (iPLA2). No significant cross-reactivity or interference between Phospholipase A2, Calcium Independent (iPLA2) and analogues was observed.
    灵敏度
    30 pg/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Calcium Independent (iPLA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Calcium Independent (iPLA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Calcium Independent (iPLA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Calcium Independent (iPLA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2, Calcium Independent (iPLA2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2, Calcium Independent (iPLA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all PNPLA2 ELISA试剂盒
    PNPLA2 (Calcium Independent Phospholipase A2 (PNPLA2))
    别名
    Phospholipase A2, Calcium Independent (PNPLA2 产品)
    别名
    1110001C14Rik ELISA Kit, ATGL ELISA Kit, PEDF-R ELISA Kit, TTS-2.2 ELISA Kit, TTS2 ELISA Kit, iPLA2zeta ELISA Kit, BB112799 ELISA Kit, PNPLA9 ELISA Kit, iPLA(2)beta ELISA Kit, iPLA2 ELISA Kit, iPLA2beta ELISA Kit, patatin like phospholipase domain containing 2 ELISA Kit, phospholipase A2, group VI ELISA Kit, PNPLA2 ELISA Kit, Pla2g6 ELISA Kit
    背景

    Gene Name: Phospholipase A2, Calcium Independent

    Gene Aliases: PLA2G6, CaI-PLA2, GVI, INAD1, PNPLA9, Ca2+ Independent PLA2, 85 kDa Calcium-Independent Phospholipase A2, Phospholipase A2,group VI(Cytosolic,Calcium-Independent)

    基因ID
    8398
    UniProt
    O60733
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