PEPCK ELISA 试剂盒
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- 抗原 See all PEPCK ELISA试剂盒
- PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56 ng/mL - 100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2). No significant cross-reactivity or interference between Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2) and analogues was observed.
- 灵敏度
- 0.74 ng/mL
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phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) ELISA Kit
PEPCK 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) ELISA KitPEPCK 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all PEPCK ELISA试剂盒
- PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))
- 别名
- Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PEPCK 产品)
- 别名
- PEPCK ELISA Kit, PEPCK-M ELISA Kit, PEPCK2 ELISA Kit, 1810010O14Rik ELISA Kit, 9130022B02Rik ELISA Kit, pck ELISA Kit, zgc:77867 ELISA Kit, id:ibd5157 ELISA Kit, pck2 ELISA Kit, pck2.S ELISA Kit, phosphoenolpyruvate carboxykinase 2, mitochondrial ELISA Kit, phosphoenolpyruvate carboxykinase 2 (mitochondrial) ELISA Kit, phosphoenolpyruvate carboxykinase 2 (mitochondrial) L homeolog ELISA Kit, PCK2 ELISA Kit, Pck2 ELISA Kit, pck2 ELISA Kit, pck2.L ELISA Kit
- 背景
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Gene Name: Phosphoenolpyruvate Carboxykinase 2, Mitochondrial
Gene Aliases: PEPCK, PEPCK2
- 基因ID
- 5106
- UniProt
- Q16822
- 途径
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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