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Phenylalanine Hydroxylase ELISA 试剂盒

适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
产品编号 ABIN5657754
发货至: 中国
  • 抗原 See all Phenylalanine Hydroxylase ELISA试剂盒
    Phenylalanine Hydroxylase
    适用
    • 4
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    1.56 ng/mL - 100 ng/mL
    最低检测浓度
    1.56 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Phenylalanine Hydroxylase (PAH). No significant cross-reactivity or interference between Phenylalanine Hydroxylase (PAH) and analogues was observed.
    灵敏度
    0.64 ng/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phenylalanine Hydroxylase (PAH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phenylalanine Hydroxylase (PAH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phenylalanine Hydroxylase (PAH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phenylalanine Hydroxylase (PAH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phenylalanine Hydroxylase (PAH) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phenylalanine Hydroxylase (PAH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all Phenylalanine Hydroxylase ELISA试剂盒
    Phenylalanine Hydroxylase
    Abstract
    Phenylalanine Hydroxylase 产品
    别名
    cb877 ELISA Kit, wu:fb02f10 ELISA Kit, zgc:65981 ELISA Kit, GB18494 ELISA Kit, PAH ELISA Kit, pku ELISA Kit, pku1 ELISA Kit, DDBDRAFT_0206188 ELISA Kit, DDBDRAFT_0231664 ELISA Kit, DDB_0206188 ELISA Kit, DDB_0231664 ELISA Kit, AW106920 ELISA Kit, PH ELISA Kit, PKU ELISA Kit, PKU1 ELISA Kit, phenylalanine-4-hydroxylase ELISA Kit, phenylalanine hydroxylase ELISA Kit, protein henna ELISA Kit, phenylalanine 4-monooxygenase ELISA Kit, pah ELISA Kit, LOC408622 ELISA Kit, PAH ELISA Kit, CpipJ_CPIJ002149 ELISA Kit, Pah ELISA Kit
    物质类
    Chemical
    背景

    Gene Name: Phenylalanine Hydroxylase

    Gene Aliases: PKU, PKU1, Phe-4-monooxygenase

    基因ID
    5053
    UniProt
    P00439
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