PPARD ELISA 试剂盒
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- 抗原 See all PPARD ELISA试剂盒
- PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Delta (PPARd). No significant cross-reactivity or interference between Peroxisome Proliferator Activated Receptor Delta (PPARd) and analogues was observed.
- 灵敏度
- 0.115 ng/mL
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Peroxisome Proliferator-Activated Receptor delta (PPARD) ELISA Kit
PPARD 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor delta (PPARD) ELISA KitPPARD 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor delta (PPARD) ELISA KitPPARD 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor delta (PPARD) ELISA KitPPARD 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Delta (PPARd). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Delta (PPARd). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Delta (PPARd), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxisome Proliferator Activated Receptor Delta (PPARd) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Delta (PPARd) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Delta (PPARd) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all PPARD ELISA试剂盒
- PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))
- 别名
- Peroxisome Proliferator Activated Receptor Delta (PPARD 产品)
- 别名
- FAAR ELISA Kit, NR1C2 ELISA Kit, NUC1 ELISA Kit, NUCI ELISA Kit, NUCII ELISA Kit, PPARB ELISA Kit, NUC-1 ELISA Kit, Nr1c2 ELISA Kit, PPAR-beta ELISA Kit, PPAR-delta ELISA Kit, PPAR[b] ELISA Kit, PPARdelta ELISA Kit, Pparb ELISA Kit, Pparb/d ELISA Kit, PPARBETA ELISA Kit, ppard ELISA Kit, pparb ELISA Kit, PPARD ELISA Kit, PPAR-D/B ELISA Kit, peroxisome proliferator activated receptor delta ELISA Kit, peroxisome proliferator activator receptor delta ELISA Kit, peroxisome proliferator activated receptor delta S homeolog ELISA Kit, peroxisome proliferator-activated receptor delta ELISA Kit, PPARD ELISA Kit, Ppard ELISA Kit, ppard.S ELISA Kit, ppard ELISA Kit
- 背景
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Gene Name: Peroxisome Proliferator Activated Receptor Delta
Gene Aliases: FAAR, NR1C2, NUC1, NUCI, PPARB, PPARd/B, Nuclear Receptor Subfamily 1,Group C,Member 2, Nuclear hormone receptor 1, Peroxisome proliferator-activated receptor beta
- 基因ID
- 5467
- UniProt
- Q03181
- 途径
- Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Monocarboxylic Acid Catabolic Process, Smooth Muscle Cell Migration, Positive Regulation of fat Cell Differentiation
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