Peroxiredoxin 1 ELISA 试剂盒
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- 抗原 See all Peroxiredoxin 1 (PRDX1) ELISA试剂盒
- Peroxiredoxin 1 (PRDX1)
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Peroxiredoxin 1 (PRDX1). No significant cross-reactivity or interference between Peroxiredoxin 1 (PRDX1) and analogues was observed.
- 灵敏度
- 0.063 ng/mL
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Peroxiredoxin 1 (PRDX1) ELISA Kit
PRDX1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxiredoxin 1 (PRDX1) ELISA KitPRDX1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxiredoxin 1 (PRDX1) ELISA KitPRDX1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin 1 (PRDX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxiredoxin 1 (PRDX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxiredoxin 1 (PRDX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxiredoxin 1 (PRDX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxiredoxin 1 (PRDX1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxiredoxin 1 (PRDX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Peroxiredoxin 1 (PRDX1) ELISA试剂盒
- Peroxiredoxin 1 (PRDX1)
- 别名
- Peroxiredoxin 1 (PRDX1 产品)
- 别名
- MSP23 ELISA Kit, NKEF-A ELISA Kit, NKEFA ELISA Kit, PAG ELISA Kit, PAGA ELISA Kit, PAGB ELISA Kit, PRX1 ELISA Kit, PRXI ELISA Kit, TDPX2 ELISA Kit, NkefA ELISA Kit, OSF-3 ELISA Kit, OSF3 ELISA Kit, Paga ELISA Kit, PrdxI ELISA Kit, PrxI ELISA Kit, TDX2 ELISA Kit, TPxA ELISA Kit, Tdpx2 ELISA Kit, prx1 ELISA Kit, Hbp23 ELISA Kit, MGC80194 ELISA Kit, MGC84820 ELISA Kit, PRDX1 ELISA Kit, msp23 ELISA Kit, nkefa ELISA Kit, pag ELISA Kit, paga ELISA Kit, pagb ELISA Kit, prx-1 ELISA Kit, prxi ELISA Kit, tdpx2 ELISA Kit, hm:zehl0637 ELISA Kit, zgc:110343 ELISA Kit, TPX-2 ELISA Kit, peroxiredoxin 1 ELISA Kit, peroxiredoxin 1 S homeolog ELISA Kit, peroxiredoxin-1 pseudogene ELISA Kit, PRDX1 ELISA Kit, Prdx1 ELISA Kit, prdx1.S ELISA Kit, prdx1 ELISA Kit, LOC100350232 ELISA Kit
- 背景
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Gene Name: Peroxiredoxin 1
Gene Aliases: MSP23, NKEFA, PAG, PAGA, PAGB, TDPX2, Natural killer cell-enhancing factor A, Proliferation-associated, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2
- 途径
- p53 Pathway, EGFR Signaling Pathway, CXCR4-mediated Signaling Events
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