Parkin ELISA 试剂盒
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- 抗原 See all Parkin (PARK2) ELISA试剂盒
- Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Parkinson Disease Protein 2 (PARK2). No significant cross-reactivity or interference between Parkinson Disease Protein 2 (PARK2) and analogues was observed.
- 灵敏度
- 0.053 ng/mL
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Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) ELISA Kit
PARK2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Parkinson Disease Protein 2 (PARK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Parkinson Disease Protein 2 (PARK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Parkinson Disease Protein 2 (PARK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Parkinson Disease Protein 2 (PARK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parkinson Disease Protein 2 (PARK2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parkinson Disease Protein 2 (PARK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Parkin (PARK2) ELISA试剂盒
- Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))
- 别名
- Parkinson Disease Protein 2 (PARK2 产品)
- 别名
- CG10523 ELISA Kit, Dmel\\CG10523 ELISA Kit, Dpark ELISA Kit, SD01679 ELISA Kit, dpk ELISA Kit, AR-JP ELISA Kit, LPRS2 ELISA Kit, PDJ ELISA Kit, PRKN ELISA Kit, Park ELISA Kit, Prkn ELISA Kit, si:ch211-123f21.1 ELISA Kit, zgc:112390 ELISA Kit, pdr-1 ELISA Kit, PARK2 ELISA Kit, parkin ELISA Kit, parkin RBR E3 ubiquitin protein ligase ELISA Kit, Parkinson disease (autosomal recessive, juvenile) 2, parkin ELISA Kit, park ELISA Kit, PRKN ELISA Kit, Prkn ELISA Kit, prkn ELISA Kit, CpipJ_CPIJ014867 ELISA Kit, Park2 ELISA Kit
- 背景
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Gene Name: Parkinson Disease Protein 2
Gene Aliases: AR-JP, LPRS2, PDJ, PRKN, Parkin, Autosomal Recessive,Juvenile, E3 ubiquitin-protein ligase parkin
- 基因ID
- 5071
- UniProt
- O60260
- 途径
- Autophagy, Ubiquitin Proteasome Pathway
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