PAK4 CLIA Kit
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- 抗原 See all PAK4 CLIA Kits
- PAK4 (P21-Activated Kinase 4 (PAK4))
- 适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 625.00 pg/mL - 40000 pg/mL
- 最低检测浓度
- 625.00 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of p21 Protein Activated Kinase 4 (PAK4). No significant cross-reactivity or interference between p21 Protein Activated Kinase 4 (PAK4) and analogues was observed.
- 灵敏度
- 20.86 pg/mL
- Top Product
- Discover our top product PAK4 ELISA Kit
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P21-Activated Kinase 4 (PAK4) ELISA Kit
PAK4 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
P21-Activated Kinase 4 (PAK4) ELISA KitPAK4 适用: 人 Colorimetric Sandwich ELISA 0.156-10 ng/mL Cell Culture Cells, Plasma, Serum
P21-Activated Kinase 4 (PAK4) ELISA KitPAK4 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to p21 Protein Activated Kinase 4 (PAK4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to p21 Protein Activated Kinase 4 (PAK4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the p21 Protein Activated Kinase 4 (PAK4) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level p21 Protein Activated Kinase 4 (PAK4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level p21 Protein Activated Kinase 4 (PAK4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all PAK4 CLIA Kits
- PAK4 (P21-Activated Kinase 4 (PAK4))
- 别名
- p21 Protein Activated Kinase 4 (PAK4 产品)
- 别名
- 5730488L07Rik CLIA Kit, AW555722 CLIA Kit, mKIAA1142 CLIA Kit, im:5916170 CLIA Kit, wu:fd12b01 CLIA Kit, zgc:92014 CLIA Kit, p21 (RAC1) activated kinase 4 CLIA Kit, p21 protein (Cdc42/Rac)-activated kinase 4 CLIA Kit, PAK4 CLIA Kit, Pak4 CLIA Kit, pak4 CLIA Kit
- 背景
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Gene Name: p21 Protein Activated Kinase 4
Gene Aliases: Serine/threonine-protein kinase PAK 4
- 基因ID
- 10298
- UniProt
- O96013
- 途径
- RTK signaling
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