NPM1 ELISA 试剂盒
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- 抗原 See all NPM1 ELISA试剂盒
- NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Nucleophosmin (NPM). No significant cross-reactivity or interference between Nucleophosmin (NPM) and analogues was observed.
- 灵敏度
- 0.119 ng/mL
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Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) ELISA Kit
NPM1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) ELISA KitNPM1 适用: 人 Colorimetric Sandwich ELISA 23.5 pg/mL - 1500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleophosmin (NPM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleophosmin (NPM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleophosmin (NPM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nucleophosmin (NPM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleophosmin (NPM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleophosmin (NPM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all NPM1 ELISA试剂盒
- NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))
- 别名
- Nucleophosmin (NPM1 产品)
- 别名
- B23 ELISA Kit, NPM ELISA Kit, NO38 ELISA Kit, Npm ELISA Kit, B23NP ELISA Kit, npm4 ELISA Kit, npm ELISA Kit, CG7917 ELISA Kit, CRP1 ELISA Kit, Crp1 ELISA Kit, Dmel\\CG7917 ELISA Kit, dNLP ELISA Kit, dNLP-S ELISA Kit, p22 ELISA Kit, npm1 ELISA Kit, sb:cb487 ELISA Kit, nucleophosmin 1 ELISA Kit, nucleophosmin (nucleolar phosphoprotein B23, numatrin) L homeolog ELISA Kit, nucleophosmin 1b ELISA Kit, Nucleophosmin ELISA Kit, nucleophosmin ELISA Kit, nucleophosmin (nucleolar phosphoprotein B23, numatrin) ELISA Kit, Nucleoplasmin ELISA Kit, nucleophosmin 1a ELISA Kit, NPM1 ELISA Kit, Npm1 ELISA Kit, npm1.L ELISA Kit, npm1b ELISA Kit, npm ELISA Kit, Nlp ELISA Kit, npm1a ELISA Kit
- 背景
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Gene Name: Nucleophosmin
Gene Aliases: B23, NPM1, Nucleolar Phosphoprotein B23, Numatrin, Nucleophosmin/Nucleoplasmin Family,Member 1, Nucleolar protein NO38
- 基因ID
- 4869
- UniProt
- P06748
- 途径
- Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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