Neuropilin 1 ELISA 试剂盒
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- 抗原 See all Neuropilin 1 (NRP1) ELISA试剂盒
- Neuropilin 1 (NRP1)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5 pg/mL - 4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Neuropilin 1 (NRP1). No significant cross-reactivity or interference between Neuropilin 1 (NRP1) and analogues was observed.
- 灵敏度
- 26.2 pg/mL
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Neuropilin 1 (NRP1) ELISA Kit
NRP1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Neuropilin 1 (NRP1) ELISA KitNRP1 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Neuropilin 1 (NRP1) ELISA KitNRP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Neuropilin 1 (NRP1) ELISA KitNRP1 适用: 大鼠 AA 22-854 Colorimetric Sandwich ELISA 780-50.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Neuropilin 1 (NRP1) ELISA KitNRP1 适用: 人 AA 22-644 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Neuropilin 1 (NRP1) ELISA KitNRP1 适用: 小鼠 AA 22-856 Colorimetric Sandwich ELISA 780-50.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuropilin 1 (NRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuropilin 1 (NRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuropilin 1 (NRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuropilin 1 (NRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropilin 1 (NRP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropilin 1 (NRP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Neuropilin 1 (NRP1) ELISA试剂盒
- Neuropilin 1 (NRP1)
- 别名
- Neuropilin 1 (NRP1 产品)
- 别名
- NRP1 ELISA Kit, cd304 ELISA Kit, neuropilin ELISA Kit, np-1 ELISA Kit, npn-1 ELISA Kit, nrp ELISA Kit, nrp-1 ELISA Kit, vegf165r ELISA Kit, BDCA4 ELISA Kit, CD304 ELISA Kit, NP1 ELISA Kit, NRP ELISA Kit, VEGF165R ELISA Kit, C530029I03 ELISA Kit, NP-1 ELISA Kit, NPN-1 ELISA Kit, Npn1 ELISA Kit, Nrp ELISA Kit, neuropilin 1 ELISA Kit, neuropilin 1 L homeolog ELISA Kit, NRP1 ELISA Kit, nrp1 ELISA Kit, Nrp1 ELISA Kit, nrp1.L ELISA Kit
- 背景
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Gene Name: Neuropilin 1
Gene Aliases: CD304, BDCA4, VEGF165R, Vascular endothelial cell growth factor 165 receptor
- 基因ID
- 8829
- UniProt
- O14786
- 途径
- Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals
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