Neurogranin ELISA 试剂盒
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- 抗原 See all Neurogranin (NRGN) ELISA试剂盒
- Neurogranin (NRGN)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 123.5 pg/mL - 10000 pg/mL
- 最低检测浓度
- 123.5 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Neurogranin (NRGN). No significant cross-reactivity or interference between Neurogranin (NRGN) and analogues was observed.
- 灵敏度
- 54.2 pg/mL
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- Discover our best selling NRGN ELISA Kit
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- Discover our top product NRGN ELISA Kit
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Neurogranin (NRGN) ELISA Kit
NRGN 适用: 人 Colorimetric Competition ELISA 16 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neurogranin (NRGN) ELISA KitNRGN 适用: 人, 小鼠, 大鼠 Colorimetric Competition ELISA 16 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neurogranin (NRGN) ELISA KitNRGN 适用: 人 Colorimetric Sandwich ELISA 18.75 ng/mL - 1200 ng/mL Plasma, Serum, Tissue Homogenate
Neurogranin (NRGN) ELISA KitNRGN 适用: 人, 小鼠, 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neurogranin (NRGN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neurogranin (NRGN) and unlabeled Neurogranin (NRGN) (Standards or samples) with the pre-coated antibody specific to Neurogranin (NRGN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neurogranin (NRGN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neurogranin (NRGN) in the sample.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurogranin (NRGN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurogranin (NRGN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Neurogranin (NRGN) ELISA试剂盒
- Neurogranin (NRGN)
- 别名
- Neurogranin (NRGN 产品)
- 别名
- RC3 ELISA Kit, hng ELISA Kit, 0710001B06Rik ELISA Kit, AI838505 ELISA Kit, NG ELISA Kit, NG/RC3 ELISA Kit, Pss1 ELISA Kit, R75334 ELISA Kit, zgc:158686 ELISA Kit, neurogranin ELISA Kit, neurogranin (protein kinase C substrate, RC3) b ELISA Kit, NRGN ELISA Kit, Nrgn ELISA Kit, nrgnb ELISA Kit
- 背景
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Gene Name: Neurogranin
Gene Aliases: Ng, NEUG, Protein Kinase C Substrate RC3
- 基因ID
- 4900
- UniProt
- Q92686
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