MME ELISA 试剂盒
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- 抗原 See all MME ELISA试剂盒
- MME (Membrane Metallo-Endopeptidase (MME))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cerebrospinal Fluid, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Neprilysin (NEP). No significant cross-reactivity or interference between Neprilysin (NEP) and analogues was observed.
- 灵敏度
- 11.9 pg/mL
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Membrane Metallo-Endopeptidase (MME) ELISA Kit
MME 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 人 AA 52-750 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 小鼠 AA 52-750 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 小鼠 Colorimetric Sandwich ELISA 61-15000 pg/mL Cell Culture Supernatant, Plasma, Serum
SensoLyte® 520 Neprilysin Activity Assay KitAct, FRET Fluorometric
Membrane Metallo-Endopeptidase (MME) ELISA KitMME 适用: 人 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neprilysin (NEP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neprilysin (NEP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neprilysin (NEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neprilysin (NEP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neprilysin (NEP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neprilysin (NEP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all MME ELISA试剂盒
- MME (Membrane Metallo-Endopeptidase (MME))
- 别名
- Neprilysin (MME 产品)
- 别名
- CALLA ELISA Kit, CD10 ELISA Kit, NEP ELISA Kit, SFE ELISA Kit, 6030454K05Rik ELISA Kit, C85356 ELISA Kit, Nep ELISA Kit, neprilysin ELISA Kit, membrane metalloendopeptidase ELISA Kit, membrane metallo endopeptidase ELISA Kit, membrane metallo-endopeptidase ELISA Kit, MME ELISA Kit, Mme ELISA Kit
- 物质类
- Chemical
- 背景
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Gene Name: Neprilysin
Gene Aliases: CD10, MME, CALLA, SFE, Atriopeptidase, Enkephalinase, Skin fibroblast elastase, Common Acute Lymphoblastic Leukemia Antigen, Membrane Metallo-Endopeptidase
- 基因ID
- 4311
- UniProt
- P08473
- 途径
- RTK signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration
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