MIOX ELISA 试剂盒
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- 抗原 See all MIOX ELISA试剂盒
- MIOX (Myo-Inositol Oxygenase (MIOX))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Myo Inositol Oxygenase (MIOX). No significant cross-reactivity or interference between Myo Inositol Oxygenase (MIOX) and analogues was observed.
- 灵敏度
- 0.069 ng/mL
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Myo-Inositol Oxygenase (MIOX) ELISA Kit
MIOX 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Myo-Inositol Oxygenase (MIOX) ELISA KitMIOX 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myo Inositol Oxygenase (MIOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myo Inositol Oxygenase (MIOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myo Inositol Oxygenase (MIOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myo Inositol Oxygenase (MIOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myo Inositol Oxygenase (MIOX) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myo Inositol Oxygenase (MIOX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all MIOX ELISA试剂盒
- MIOX (Myo-Inositol Oxygenase (MIOX))
- 别名
- Myo Inositol Oxygenase (MIOX 产品)
- 别名
- ALDRL6 ELISA Kit, 0610009I10Rik ELISA Kit, AI314022 ELISA Kit, Aldrl6 ELISA Kit, C85427 ELISA Kit, RSOR ELISA Kit, MGC82300 ELISA Kit, MGC86377 ELISA Kit, aldrl6 ELISA Kit, zgc:114168 ELISA Kit, DDBDRAFT_0188751 ELISA Kit, DDBDRAFT_0235367 ELISA Kit, DDB_0188751 ELISA Kit, DDB_0235367 ELISA Kit, MI oxygenase ELISA Kit, myo-inositol oxygenase ELISA Kit, myo-inositol oxygenase L homeolog ELISA Kit, inositol oxygenase 1 ELISA Kit, inositol oxygenase ELISA Kit, Myo-inositol oxygenase ELISA Kit, MIOX ELISA Kit, Miox ELISA Kit, miox.L ELISA Kit, miox ELISA Kit, MGG_09462 ELISA Kit, CNF03950 ELISA Kit, CC1G_10713 ELISA Kit, SCHCODRAFT_258535 ELISA Kit, CGB_F1290W ELISA Kit, mioX ELISA Kit, LOC100101177 ELISA Kit, LOC107131132 ELISA Kit
- 背景
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Gene Name: Myo Inositol Oxygenase
Gene Aliases: ALDRL6, KSP32, RSOR, Kidney-Specific Protein 32, Aldehyde Reductase(Aldose Reductase)Like 6, Renal-specific oxidoreductase, Myo-inositol oxygenase
- 途径
- Inositol Metabolic Process
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