MCL-1 ELISA 试剂盒
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- 抗原 See all MCL-1 (MCL1) ELISA试剂盒
- MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). No significant cross-reactivity or interference between Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) and analogues was observed.
- 灵敏度
- 0.127 ng/mL
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Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) ELISA Kit
MCL1 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) ELISA KitMCL1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) ELISA KitMCL1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all MCL-1 (MCL1) ELISA试剂盒
- MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
- 别名
- Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1 产品)
- 别名
- BCL2L3 ELISA Kit, EAT ELISA Kit, MCL1-ES ELISA Kit, MCL1L ELISA Kit, MCL1S ELISA Kit, Mcl-1 ELISA Kit, TM ELISA Kit, bcl2-L-3 ELISA Kit, mcl1/EAT ELISA Kit, AW556805 ELISA Kit, mcl-1 ELISA Kit, fb72f02 ELISA Kit, wu:fb70a08 ELISA Kit, wu:fb72f02 ELISA Kit, zfMLP1 ELISA Kit, zfMcl-1a ELISA Kit, zgc:109835 ELISA Kit, zgc:109763 ELISA Kit, E3 ELISA Kit, E3-2 ELISA Kit, RNE3 ELISA Kit, RNU107 ELISA Kit, SNORA63A ELISA Kit, elF-4AII ELISA Kit, MCL1, BCL2 family apoptosis regulator ELISA Kit, myeloid cell leukemia sequence 1 ELISA Kit, MCL1, BCL2 family apoptosis regulator a ELISA Kit, MCL1, BCL2 family apoptosis regulator b ELISA Kit, small nucleolar RNA, H/ACA box 63 ELISA Kit, MCL1 ELISA Kit, Mcl1 ELISA Kit, mcl1a ELISA Kit, mcl1b ELISA Kit, SNORA63 ELISA Kit
- 背景
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Gene Name: Myeloid Cell Leukemia Sequence 1, Bcl2 Related
Gene Aliases: EAT, MCL1L, MCL1S, BCL2L3, TM, Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT
- 基因ID
- 4170
- UniProt
- Q07820
- 途径
- MAPK Pathway
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