SMAD2 ELISA 试剂盒
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- 抗原 See all SMAD2 ELISA试剂盒
- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Mothers Against Decapentaplegic Homolog 2 (Smad2). No significant cross-reactivity or interference between Mothers Against Decapentaplegic Homolog 2 (Smad2) and analogues was observed.
- 灵敏度
- 0.057 ng/mL
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SMAD, Mothers Against DPP Homolog 2 (SMAD2) ELISA Kit
SMAD2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
SMAD, Mothers Against DPP Homolog 2 (SMAD2) ELISA KitSMAD2 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
SMAD, Mothers Against DPP Homolog 2 (SMAD2) ELISA KitSMAD2 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
SMAD, Mothers Against DPP Homolog 2 (SMAD2) ELISA KitSMAD2 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
SMAD, Mothers Against DPP Homolog 2 (SMAD2) ELISA KitSMAD2 适用: 人, 大鼠, 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 2 (Smad2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mothers Against Decapentaplegic Homolog 2 (Smad2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mothers Against Decapentaplegic Homolog 2 (Smad2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mothers Against Decapentaplegic Homolog 2 (Smad2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 2 (Smad2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 2 (Smad2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all SMAD2 ELISA试剂盒
- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
- 别名
- Mothers Against Decapentaplegic Homolog 2 (SMAD2 产品)
- 别名
- JV18 ELISA Kit, JV18-1 ELISA Kit, MADH2 ELISA Kit, MADR2 ELISA Kit, hMAD-2 ELISA Kit, hSMAD2 ELISA Kit, jv18 ELISA Kit, Xmad2 ELISA Kit, madh2 ELISA Kit, madr2 ELISA Kit, XSmad2 ELISA Kit, hmad-2 ELISA Kit, hsmad2 ELISA Kit, jv18-1 ELISA Kit, smad-2 ELISA Kit, fj43c06 ELISA Kit, wu:fj43c06 ELISA Kit, 7120426M23Rik ELISA Kit, Madh2 ELISA Kit, Madr2 ELISA Kit, Smad-2 ELISA Kit, mMad2 ELISA Kit, MAD homolog 2 ELISA Kit, Mothers against DPP homolog 2 ELISA Kit, SMAD 2 ELISA Kit, Smad2 ELISA Kit, SMAD family member 2 ELISA Kit, SMAD family member 2-Z ELISA Kit, SMAD family member 2 S homeolog ELISA Kit, SMAD2 ELISA Kit, SMAD2Z ELISA Kit, smad2 ELISA Kit, smad2.S ELISA Kit, Smad2 ELISA Kit
- 背景
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Gene Name: Mothers Against Decapentaplegic Homolog 2
Gene Aliases: JV18, JV18-1, MADH2, MADR2, HMAD-2, HSMAD2, Mad-related protein 2
- 途径
- Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
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