MEK1 ELISA 试剂盒
-
- 抗原 See all MEK1 (MAP2K1) ELISA试剂盒
- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Mitogen Activated Protein Kinase Kinase 1 (MAP2K1). No significant cross-reactivity or interference between Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) and analogues was observed.
- 灵敏度
- 0.056 ng/mL
- Featured
- Discover our best selling MAP2K1 ELISA Kit
- Top Product
- Discover our top product MAP2K1 ELISA Kit
-
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA Kit
MAP2K1 适用: 人, 大鼠, 小鼠 pSer217 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA KitMAP2K1 适用: 人, 大鼠, 小鼠 pSer217 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA KitMAP2K1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA KitMAP2K1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA KitMAP2K1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase Kinase 1 (MAP2K1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitogen Activated Protein Kinase Kinase 1 (MAP2K1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitogen Activated Protein Kinase Kinase 1 (MAP2K1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all MEK1 (MAP2K1) ELISA试剂盒
- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
- 别名
- Mitogen Activated Protein Kinase Kinase 1 (MAP2K1 产品)
- 别名
- Mek1 ELISA Kit, CFC3 ELISA Kit, MAPKK1 ELISA Kit, MEK1 ELISA Kit, MKK1 ELISA Kit, PRKMK1 ELISA Kit, MEKK1 ELISA Kit, Prkmk1 ELISA Kit, mek-2 ELISA Kit, si:ch211-242m18.2 ELISA Kit, wu:fj56a12 ELISA Kit, wu:fj61b01 ELISA Kit, zgc:56557 ELISA Kit, ATMEK1 ELISA Kit, F20B18.180 ELISA Kit, F20B18_180 ELISA Kit, MAP kinase/ ERK kinase 1 ELISA Kit, MITOGEN ACTIVATED PROTEIN KINASE KINASE ELISA Kit, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1 ELISA Kit, NMAPKK ELISA Kit, MEK ELISA Kit, mitogen activated protein kinase kinase 1 ELISA Kit, mitogen-activated protein kinase kinase 1 ELISA Kit, mitogen-activated protein kinase kinase 1 L homeolog ELISA Kit, MAP kinase/ ERK kinase 1 ELISA Kit, Map2k1 ELISA Kit, MAP2K1 ELISA Kit, map2k1.L ELISA Kit, map2k1 ELISA Kit, MEK1 ELISA Kit
- 背景
-
Gene Name: Mitogen Activated Protein Kinase Kinase 1
Gene Aliases: MAPKK1, MEK1, MKK1, PRKMK1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1
- 基因ID
- 5604
- UniProt
- Q02750
- 途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
-
