MITF ELISA 试剂盒
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- 抗原 See all MITF ELISA试剂盒
- MITF (Microphthalmia-Associated Transcription Factor (MITF))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Microphthalmia Associated Transcription Factor (MITF). No significant cross-reactivity or interference between Microphthalmia Associated Transcription Factor (MITF) and analogues was observed.
- 灵敏度
- 0.059 ng/mL
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Microphthalmia-Associated Transcription Factor (MITF) ELISA Kit
MITF 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Microphthalmia-Associated Transcription Factor (MITF) ELISA KitMITF 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microphthalmia Associated Transcription Factor (MITF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microphthalmia Associated Transcription Factor (MITF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microphthalmia Associated Transcription Factor (MITF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microphthalmia Associated Transcription Factor (MITF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Microphthalmia Associated Transcription Factor (MITF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Microphthalmia Associated Transcription Factor (MITF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all MITF ELISA试剂盒
- MITF (Microphthalmia-Associated Transcription Factor (MITF))
- 别名
- Microphthalmia Associated Transcription Factor (MITF 产品)
- 别名
- MITF ELISA Kit, ws2a ELISA Kit, mitfa ELISA Kit, MITF-A ELISA Kit, LOC100313655 ELISA Kit, mitfb ELISA Kit, CMM8 ELISA Kit, MI ELISA Kit, WS2 ELISA Kit, WS2A ELISA Kit, bHLHe32 ELISA Kit, BCC2 ELISA Kit, Bhlhe32 ELISA Kit, Gsfbcc2 ELISA Kit, Vitiligo ELISA Kit, Wh ELISA Kit, bw ELISA Kit, mi ELISA Kit, vit ELISA Kit, MITF-H ELISA Kit, MITF-M ELISA Kit, CMI9 ELISA Kit, melanogenesis associated transcription factor ELISA Kit, microphthalmia-associated transcription factor ELISA Kit, microphthalmia-associated transcription factor S homeolog ELISA Kit, Microphthalmia ELISA Kit, MITF ELISA Kit, mitf ELISA Kit, LOC100313655 ELISA Kit, mitf.S ELISA Kit, LOC100534525 ELISA Kit, MICROP ELISA Kit, Mitf ELISA Kit
- 背景
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Gene Name: Microphthalmia Associated Transcription Factor
Gene Aliases: WS2A, MI, bHLHe32, Homolog Of Mouse Microphthalmia, Class E basic helix-loop-helix protein 32
- 途径
- Chromatin Binding
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