MEP1A ELISA 试剂盒
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- 抗原 See all MEP1A ELISA试剂盒
- MEP1A (Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 49.38 pg/mL - 4000 pg/mL
- 最低检测浓度
- 49.38 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Meprin A Alpha (MEP1a). No significant cross-reactivity or interference between Meprin A Alpha (MEP1a) and analogues was observed.
- 灵敏度
- 17.6 pg/mL
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Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) ELISA Kit
MEP1A 适用: 人 Colorimetric Competition ELISA 49.4 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) ELISA KitMEP1A 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) ELISA KitMEP1A 适用: 小鼠 Colorimetric Competition ELISA 49.4 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) ELISA KitMEP1A 适用: 大鼠 Colorimetric Competition ELISA 49.4 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) ELISA KitMEP1A 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Meprin A Alpha (MEP1a) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Meprin A Alpha (MEP1a) and unlabeled Meprin A Alpha (MEP1a) (Standards or samples) with the pre-coated antibody specific to Meprin A Alpha (MEP1a). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Meprin A Alpha (MEP1a) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Meprin A Alpha (MEP1a) in the sample.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Meprin A Alpha (MEP1a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Meprin A Alpha (MEP1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all MEP1A ELISA试剂盒
- MEP1A (Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A))
- 别名
- Meprin A Alpha (MEP1A 产品)
- 别名
- AI098089 ELISA Kit, AW107200 ELISA Kit, Mep-1 ELISA Kit, Mep-1a ELISA Kit, Mep1 ELISA Kit, PPHA ELISA Kit, meprin A subunit alpha ELISA Kit, meprin 1 alpha ELISA Kit, Mep1a ELISA Kit, MEP1A ELISA Kit
- 背景
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Gene Name: Meprin A Alpha
Gene Aliases: PPHA, PABA Peptide Hydrolase, Endopeptidase-2, N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha, PABA Peptide Hydrolase
- 基因ID
- 17287
- UniProt
- P28825
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