CD46 ELISA 试剂盒
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- 抗原 See all CD46 ELISA试剂盒
- CD46
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 12.5 pg/mL - 800 pg/mL
- 最低检测浓度
- 12.5 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Saliva, Serum, Urine
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Membrane Cofactor Protein (MCP). No significant cross-reactivity or interference between Membrane Cofactor Protein (MCP) and analogues was observed.
- 灵敏度
- 4.8 pg/mL
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- Discover our top product CD46 ELISA Kit
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CD46 (CD46) ELISA Kit
CD46 适用: 人 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Plasma, Saliva, Serum, Urine
CD46 (CD46) ELISA KitCD46 适用: Cow Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
CD46 (CD46) ELISA KitCD46 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
CD46 (CD46) ELISA KitCD46 适用: 人 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Membrane Cofactor Protein (MCP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Membrane Cofactor Protein (MCP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Membrane Cofactor Protein (MCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Membrane Cofactor Protein (MCP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Membrane Cofactor Protein (MCP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Membrane Cofactor Protein (MCP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all CD46 ELISA试剂盒
- CD46
- 别名
- Membrane Cofactor Protein (CD46 产品)
- 别名
- AHUS2 ELISA Kit, MCP ELISA Kit, MIC10 ELISA Kit, TLX ELISA Kit, TRA2.10 ELISA Kit, Mcp ELISA Kit, CD46 ELISA Kit, CD46 molecule ELISA Kit, CD46 antigen, complement regulatory protein ELISA Kit, CD46 molecule, complement regulatory protein ELISA Kit, membrane cofactor protein-like ELISA Kit, CD46 ELISA Kit, Cd46 ELISA Kit, LOC100341359 ELISA Kit, LOC103789310 ELISA Kit
- 背景
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Gene Name: Membrane Cofactor Protein
Gene Aliases: CD46, MIC10, TLX, TRA2, Trophoblast leukocyte common antigen, CD46 Molecule,Complement Regulatory Protein, CD46 Molecule,Complement Regulatory protein
- 基因ID
- 4179
- UniProt
- P15529
- 途径
- Regulation of Actin Filament Polymerization
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