MCAM ELISA 试剂盒
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- 抗原 See all MCAM ELISA试剂盒
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.625 ng/mL - 40 ng/mL
- 最低检测浓度
- 0.625 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Melanoma Cell Adhesion Molecule (MCAM). No significant cross-reactivity or interference between Melanoma Cell Adhesion Molecule (MCAM) and analogues was observed.
- 灵敏度
- 0.251 ng/mL
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Melanoma Cell Adhesion Molecule (MCAM) ELISA Kit
MCAM 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Melanoma Cell Adhesion Molecule (MCAM) ELISA KitMCAM 适用: 人 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Melanoma Cell Adhesion Molecule (MCAM) ELISA KitMCAM 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Cell Adhesion Molecule (MCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melanoma Cell Adhesion Molecule (MCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanoma Cell Adhesion Molecule (MCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Melanoma Cell Adhesion Molecule (MCAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanoma Cell Adhesion Molecule (MCAM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanoma Cell Adhesion Molecule (MCAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all MCAM ELISA试剂盒
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
- 别名
- Melanoma Cell Adhesion Molecule (MCAM 产品)
- 别名
- CD146 ELISA Kit, MUC18 ELISA Kit, Muc18 ELISA Kit, 1-gicerin ELISA Kit, AV025631 ELISA Kit, CD149 ELISA Kit, s-endo ELISA Kit, s-gicerin ELISA Kit, cd146 ELISA Kit, gicerin ELISA Kit, id:ibd3512 ELISA Kit, mcam ELISA Kit, MCAM ELISA Kit, melanoma cell adhesion molecule ELISA Kit, melanoma cell adhesion molecule b ELISA Kit, MCAM ELISA Kit, Mcam ELISA Kit, mcamb ELISA Kit
- 背景
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Gene Name: Melanoma Cell Adhesion Molecule
Gene Aliases: CD146, MUC18, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, S-endo 1 endothelial-associated antigen
- 基因ID
- 84004
- UniProt
- Q8R2Y2
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