MBL2 ELISA 试剂盒
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- 抗原 See all MBL2 ELISA试剂盒
- MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Mannose Binding Lectin (MBL). No significant cross-reactivity or interference between Mannose Binding Lectin (MBL) and analogues was observed.
- 灵敏度
- 0.063 ng/mL
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Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA Kit
MBL2 适用: 人 Colorimetric Sandwich ELISA 1.563-100 ng/mL Serum
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 小鸡 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 人 AA 21-248 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 小鼠 AA 19-244 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 小鼠 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Binding Lectin (MBL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Binding Lectin (MBL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Binding Lectin (MBL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose Binding Lectin (MBL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all MBL2 ELISA试剂盒
- MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))
- 别名
- Mannose Binding Lectin (MBL2 产品)
- 别名
- COLEC1 ELISA Kit, HSMBPC ELISA Kit, MBL ELISA Kit, MBL2D ELISA Kit, MBP ELISA Kit, MBP-C ELISA Kit, MBP1 ELISA Kit, MBPD ELISA Kit, pMBP-27 ELISA Kit, mbl2 ELISA Kit, MBL1 ELISA Kit, cMBl ELISA Kit, collectin ELISA Kit, Ab2-001 ELISA Kit, Ab2-011 ELISA Kit, L-MBP ELISA Kit, MBL-C ELISA Kit, COLEC2 ELISA Kit, mbl ELISA Kit, etID42583.2 ELISA Kit, fb68b07 ELISA Kit, hbl3 ELISA Kit, wu:fb68b07 ELISA Kit, zgc:109836 ELISA Kit, mannose binding lectin 2 ELISA Kit, Mannose-binding protein C ELISA Kit, mannose-binding lectin (protein C) 2, soluble ELISA Kit, mannose-binding lectin (protein C) 2 ELISA Kit, mannose-binding lectin family member 3, pseudogene ELISA Kit, MBL2 ELISA Kit, mbl2 ELISA Kit, Mbl2 ELISA Kit, MBL3P ELISA Kit
- 背景
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Gene Name: Mannose Binding Lectin
Gene Aliases: MBL2, COLEC1, HSMBPC, MBP1, MBP, Collectin-1, Mannose-binding protein C, Mannan Binding Protein, Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
- 基因ID
- 4153
- UniProt
- P11226
- 途径
- Complement System, Positive Regulation of Immune Effector Process
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