LEFTY2 ELISA 试剂盒
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- 抗原 See all LEFTY2 ELISA试剂盒
- LEFTY2 (Left-Right Determination Factor 2 (LEFTY2))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.62 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.62 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Left/Right Determination Factor 2 (LEFTY2). No significant cross-reactivity or interference between Left/Right Determination Factor 2 (LEFTY2) and analogues was observed.
- 灵敏度
- 5.5 pg/mL
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Left-Right Determination Factor 2 (LEFTY2) ELISA Kit
LEFTY2 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Left-Right Determination Factor 2 (LEFTY2) ELISA KitLEFTY2 适用: 人 Colorimetric Sandwich ELISA 7.8-500 pg/mL
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 2 (LEFTY2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 2 (LEFTY2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Left/Right Determination Factor 2 (LEFTY2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Left/Right Determination Factor 2 (LEFTY2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Left/Right Determination Factor 2 (LEFTY2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Left/Right Determination Factor 2 (LEFTY2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all LEFTY2 ELISA试剂盒
- LEFTY2 (Left-Right Determination Factor 2 (LEFTY2))
- 别名
- Left/Right Determination Factor 2 (LEFTY2 产品)
- 别名
- EBAF ELISA Kit, LEFTA ELISA Kit, LEFTYA ELISA Kit, TGFB4 ELISA Kit, AI450052 ELISA Kit, Ebaf ELISA Kit, Leftb ELISA Kit, Stra3 ELISA Kit, Tgfb4 ELISA Kit, lefty ELISA Kit, lefty-1 ELISA Kit, atv2 ELISA Kit, cb720 ELISA Kit, fe38b03 ELISA Kit, wu:fe38b03 ELISA Kit, 6030463A22Rik ELISA Kit, AV214969 ELISA Kit, Lefta ELISA Kit, Ebaf2 ELISA Kit, left-right determination factor 2 ELISA Kit, left right determination factor 1 ELISA Kit, lefty2 ELISA Kit, Left-right determination factor 2 ELISA Kit, left-right determination factor 2-like ELISA Kit, LEFTY2 ELISA Kit, Lefty1 ELISA Kit, lft2 ELISA Kit, Lefty2 ELISA Kit, LOC699676 ELISA Kit, LOC100232580 ELISA Kit
- 背景
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Gene Name: Left/Right Determination Factor 2
Gene Aliases: TGFB4, EBAF, LEFTA, LEFTYA, Endometrial Bleeding Associated Factor, Left-right determination factor A, Transforming growth factor beta-4
- 基因ID
- 320202
- UniProt
- P57785
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