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LEFTY1 CLIA Kit

LEFTY1 适用: 小鼠 Chemiluminescent Sandwich ELISA 156.25 pg/mL - 10000 pg/mL Plasma, Serum, Tissue Homogenate
产品编号 ABIN5656347
发货至: 中国
  • 抗原 See all LEFTY1 CLIA Kits
    LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))
    适用
    • 4
    • 3
    • 3
    小鼠
    检测方法
    Chemiluminescent
    实验类型
    Sandwich ELISA
    检测范围
    156.25 pg/mL - 10000 pg/mL
    最低检测浓度
    156.25 pg/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Left/Right Determination Factor 1 (LEFTY1). No significant cross-reactivity or interference between Left/Right Determination Factor 1 (LEFTY1) and analogues was observed.
    灵敏度
    5.61 pg/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    2 - 3 h
    板类型
    Pre-coated
    实验流程
    The microplate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 1 (LEFTY1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 1 (LEFTY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Left/Right Determination Factor 1 (LEFTY1) level in the sample or standard.,
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all LEFTY1 CLIA Kits
    LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))
    别名
    Left/Right Determination Factor 1 (LEFTY1 产品)
    别名
    atv CLIA Kit, cb73 CLIA Kit, antivin CLIA Kit, ik:tdsubc_2b12 CLIA Kit, xx:tdsubc_2b12 CLIA Kit, LEFTY1 CLIA Kit, AI450052 CLIA Kit, Ebaf CLIA Kit, Leftb CLIA Kit, Stra3 CLIA Kit, Tgfb4 CLIA Kit, lefty CLIA Kit, lefty-1 CLIA Kit, RGD1561867 CLIA Kit, LEFTB CLIA Kit, LEFTYB CLIA Kit, lefty1 CLIA Kit, signaling molecule lefty1 CLIA Kit, left-right determination factor 1 CLIA Kit, left right determination factor 1 CLIA Kit, lft1 CLIA Kit, lefty1 CLIA Kit, LOC699924 CLIA Kit, LEFTY1 CLIA Kit, LOC100408003 CLIA Kit, LOC100597378 CLIA Kit, Lefty1 CLIA Kit
    背景

    Gene Name: Left/Right Determination Factor 1

    Gene Aliases: LEFTY-B, LEFTYB, LEFT-B, LEFTB, Left Right Determination Factor B

    基因ID
    13590
    UniProt
    Q64280
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