LGALS3BP ELISA 试剂盒
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- 抗原 See all LGALS3BP ELISA试剂盒
- LGALS3BP (Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP). No significant cross-reactivity or interference between Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) and analogues was observed.
- 灵敏度
- 0.069 ng/mL
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Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP) ELISA Kit
LGALS3BP 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP) ELISA KitLGALS3BP 适用: 小鼠 Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP) ELISA KitLGALS3BP 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP) ELISA KitLGALS3BP 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP) ELISA KitLGALS3BP 适用: 人 AA 19-585 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all LGALS3BP ELISA试剂盒
- LGALS3BP (Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP))
- 别名
- Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP 产品)
- 别名
- 90K ELISA Kit, BTBD17B ELISA Kit, MAC-2-BP ELISA Kit, TANGO10B ELISA Kit, Ppicap ELISA Kit, CyCAP ELISA Kit, MAC-2BP ELISA Kit, Tango10b ELISA Kit, lgals3bp ELISA Kit, zgc:136780 ELISA Kit, MAC2BP ELISA Kit, Mac-2 BP ELISA Kit, zgc:77059 ELISA Kit, galectin 3 binding protein ELISA Kit, lectin, galactoside-binding, soluble, 3 binding protein ELISA Kit, calcium activated nucleotidase 1 ELISA Kit, lectin, galactoside-binding, soluble, 3 binding protein a ELISA Kit, lectin, galactoside-binding, soluble, 3 binding protein b ELISA Kit, LGALS3BP ELISA Kit, Lgals3bp ELISA Kit, CANT1 ELISA Kit, lgals3bpa ELISA Kit, lgals3bpb ELISA Kit
- 背景
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Gene Name: Lectin Galactoside Binding, Soluble 3 Binding Protein
Gene Aliases: MAC-2-BP, LGALS3-BP, Galectin-3-Binding Protein, L3 Antigen, Mac-2-Binding Protein, Tumor-associated antigen 90K, Serum Protein 90K, Basement membrane autoantigen p105
- 基因ID
- 19039
- UniProt
- Q07797
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