KRT6A ELISA 试剂盒
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- 抗原 See all KRT6A ELISA试剂盒
- KRT6A (Keratin 6A (KRT6A))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.62 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.62 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Keratin 6A (KRT6A). No significant cross-reactivity or interference between Keratin 6A (KRT6A) and analogues was observed.
- 灵敏度
- 6.5 pg/mL
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Keratin 6A (KRT6A) ELISA Kit
KRT6A 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Keratin 6A (KRT6A) ELISA KitKRT6A 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 6A (KRT6A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 6A (KRT6A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 6A (KRT6A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 6A (KRT6A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 6A (KRT6A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 6A (KRT6A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all KRT6A ELISA试剂盒
- KRT6A (Keratin 6A (KRT6A))
- 别名
- Keratin 6A (KRT6A 产品)
- 别名
- CK6A ELISA Kit, CK6C ELISA Kit, CK6D ELISA Kit, K6A ELISA Kit, K6C ELISA Kit, K6D ELISA Kit, KRT6C ELISA Kit, KRT6D ELISA Kit, 2310016L08Rik ELISA Kit, Krt2-6a ELISA Kit, Krt2-6c ELISA Kit, MK6a ELISA Kit, mK6[a] ELISA Kit, CK-6A ELISA Kit, Krt6a ELISA Kit, KRT6A ELISA Kit, keratin 6A ELISA Kit, similar to keratin complex 2, basic, gene 6a ELISA Kit, keratin, type II cytoskeletal 6A ELISA Kit, KRT6A ELISA Kit, Krt6a ELISA Kit, LOC683313 ELISA Kit, LOC101112304 ELISA Kit
- 背景
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Gene Name: Keratin 6A
Gene Aliases: K6A, KRT6C, KRT6D, CK6C, K6C, CK6D, K6D, Keratin 6C, Keratin 6D, Keratin, type II cytoskeletal 6A, Type-II keratin Kb6
- 基因ID
- 3853
- UniProt
- P02538
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