Keratin 10 ELISA 试剂盒
-
- 抗原 See all Keratin 10 (KRT10) ELISA试剂盒
- Keratin 10 (KRT10)
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.25 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Keratin 10 (KRT10). No significant cross-reactivity or interference between Keratin 10 (KRT10) and analogues was observed.
- 灵敏度
- 12.2 pg/mL
- Featured
- Discover our best selling KRT10 ELISA Kit
- Top Product
- Discover our top product KRT10 ELISA Kit
-
Keratin 10 (KRT10) ELISA Kit
KRT10 适用: 人 Colorimetric Sandwich ELISA 15.63 ng/mL - 1000 ng/mL Cell Culture Supernatant, Plasma, Serum
Keratin 10 (KRT10) ELISA KitKRT10 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 10 (KRT10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 10 (KRT10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 10 (KRT10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 10 (KRT10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 10 (KRT10) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 10 (KRT10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all Keratin 10 (KRT10) ELISA试剂盒
- Keratin 10 (KRT10)
- 别名
- Keratin 10 (KRT10 产品)
- 别名
- BCIE ELISA Kit, BIE ELISA Kit, CK10 ELISA Kit, EHK ELISA Kit, K10 ELISA Kit, KPP ELISA Kit, D130054E02Rik ELISA Kit, K1C1 ELISA Kit, Krt-1.10 ELISA Kit, Krt1-10 ELISA Kit, Ka10 ELISA Kit, Ker10 ELISA Kit, KRT10 ELISA Kit, KRT25 ELISA Kit, KRT27 ELISA Kit, KRT28 ELISA Kit, keratin 10 ELISA Kit, keratin 10, type I S homeolog ELISA Kit, keratin, type I cytoskeletal 25 ELISA Kit, KRT10 ELISA Kit, Krt10 ELISA Kit, krt10.S ELISA Kit, LOC101085587 ELISA Kit
- 背景
-
Gene Name: Keratin 10
Gene Aliases: K10, CK10, KPP, Cytokeratin 10, Epidermolytic Hyperkeratosis, Keratosis Palmaris Et Plantaris, Keratin, type I cytoskeletal 10
- 基因ID
- 3858
- UniProt
- P13645
-
