IARS ELISA 试剂盒
-
- 抗原 See all IARS ELISA试剂盒
- IARS (Isoleucyl-tRNA Synthetase (IARS))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Isoleucyl tRNA Synthetase (IARS). No significant cross-reactivity or interference between Isoleucyl tRNA Synthetase (IARS) and analogues was observed.
- 灵敏度
- 0.114 ng/mL
- Featured
- Discover our best selling IARS ELISA Kit
- Top Product
- Discover our top product IARS ELISA Kit
-
Isoleucyl-tRNA Synthetase (IARS) ELISA Kit
IARS 适用: 小鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Isoleucyl-tRNA Synthetase (IARS) ELISA KitIARS 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Isoleucyl tRNA Synthetase (IARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Isoleucyl tRNA Synthetase (IARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Isoleucyl tRNA Synthetase (IARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Isoleucyl tRNA Synthetase (IARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Isoleucyl tRNA Synthetase (IARS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Isoleucyl tRNA Synthetase (IARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all IARS ELISA试剂盒
- IARS (Isoleucyl-tRNA Synthetase (IARS))
- 别名
- Isoleucyl tRNA Synthetase (IARS 产品)
- 别名
- fi46h05 ELISA Kit, zgc:63790 ELISA Kit, wu:fi46h05 ELISA Kit, K19E20.18 ELISA Kit, K19E20_18 ELISA Kit, ovule abortion 2 ELISA Kit, ECK0027 ELISA Kit, ilvS ELISA Kit, JW0024 ELISA Kit, An08g06770 ELISA Kit, AO090012000505 ELISA Kit, 2510016L12Rik ELISA Kit, AI327140 ELISA Kit, AU044614 ELISA Kit, E430001P04Rik ELISA Kit, ILRS ELISA Kit, Iarsl ELISA Kit, IARS1 ELISA Kit, ILERS ELISA Kit, IRS ELISA Kit, PRO0785 ELISA Kit, isoleucyl-tRNA synthetase ELISA Kit, tRNA synthetase class I (I, L, M and V) family protein ELISA Kit, isoleucine--tRNA ligase ELISA Kit, isoleucyl-tRNA synthetase, cytoplasmic ELISA Kit, isoleucine-tRNA synthetase ELISA Kit, iars ELISA Kit, IARS ELISA Kit, OVA2 ELISA Kit, ileS ELISA Kit, MBAR_RS19160 ELISA Kit, Rmag_0340 ELISA Kit, ANI_1_964074 ELISA Kit, CHLREDRAFT_106327 ELISA Kit, EDI_049880 ELISA Kit, OE_RS08520 ELISA Kit, AOR_1_1912194 ELISA Kit, Iars ELISA Kit
- 背景
-
Gene Name: Isoleucyl tRNA Synthetase
Gene Aliases: IARS1, ILRS, OJ, IleRS, IRS, Isoleucine tRNA Ligase 1,Cytoplasmic
- 基因ID
- 3376
- UniProt
- P41252
-
